![Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry showing single asbestos fibers in the tissue (arrows). Image collected and cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-LF3-B7-D5B27-Immunohistochemistry-Paraffin-NB110-96423-img0008.jpg "Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry showing single asbestos fibers in the tissue (arrows). Image collected and cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license.")
| Reactivity | RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | LF3/B7/D5B27 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Membrane protein fraction of isolated rat glomeruli |
| Marker | Lymphatic Endothelium Marker |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PDPN |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
![Immunohistochemistry CD31/PECAM-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD31_AF3628_Immunohistochemistry_10287.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for Podoplanin Antibody (NB110-96423)Find related products by research area.
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Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege' By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int... Read full blog post. |
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Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein) Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with ... Read full blog post. |
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![Western Blot: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - analysis with recombinant human, mouse and rat soluble Podolanin. There is no cross reaction with human and mouse Podoplanin.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-LF3-B7-D5B27-Western-Blot-NB110-96423-img0004.jpg "Western Blot: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - analysis with recombinant human, mouse and rat soluble Podolanin. There is no cross reaction with human and mouse Podoplanin.")
![Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Rat cardiac lymphatic microvessels, labeled with a monoclonal antibody against rat Podoplanin (green) [A] and a polyclonal antibody against mouse LYVE-1 (red) [overlay, B]. Image was obtained at 20x magnification on a Zeiss fluorescence microscope. Scale bar = 50 um. The used protocol in short was: 1. Blockage of nonspecific binding; 2. Incubation with primary abs : anti-mouse Lyve1 (1:1000) and mouse anti- Podoplanin (1:400) for 60 min at RT; 3. Incubation with secondary abs: Donkey anti-rabbit Cy3 and Donkey anti-mouse FITC, 30 min at RT; 4. Mounting in DAPI-containing medium for cell nuclei labeling.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-LF3-B7-D5B27-Immunohistochemistry-NB110-96423-img0010.jpg "Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Rat cardiac lymphatic microvessels, labeled with a monoclonal antibody against rat Podoplanin (green) [A] and a polyclonal antibody against mouse LYVE-1 (red) [overlay, B]. Image was obtained at 20x magnification on a Zeiss fluorescence microscope. Scale bar = 50 um. The used protocol in short was: 1. Blockage of nonspecific binding; 2. Incubation with primary abs : anti-mouse Lyve1 (1:1000) and mouse anti- Podoplanin (1:400) for 60 min at RT; 3. Incubation with secondary abs: Donkey anti-rabbit Cy3 and Donkey anti-mouse FITC, 30 min at RT; 4. Mounting in DAPI-containing medium for cell nuclei labeling.")
![Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Figure: Rat cardiac lymphatic microvessels, labeled with antibodies against rat Podoplanin (A, green) and mouse LYVE-1 (B, red). Nuclear stain in blue. Double staining with anti-mouse LYVE-1 and anti-rat Podoplanin revealed a nice co-expression of both proteins in lymphatic endothelial cells. Note: The anti-mouse Lyve-1 polyclonal antibody shows a strong cross reaction with rat Lyve-1 protein.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-LF3-B7-D5B27-Immunohistochemistry-NB110-96423-img0005.jpg "Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Figure: Rat cardiac lymphatic microvessels, labeled with antibodies against rat Podoplanin (A, green) and mouse LYVE-1 (B, red). Nuclear stain in blue. Double staining with anti-mouse LYVE-1 and anti-rat Podoplanin revealed a nice co-expression of both proteins in lymphatic endothelial cells. Note: The anti-mouse Lyve-1 polyclonal antibody shows a strong cross reaction with rat Lyve-1 protein.")
![Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-LF3-B7-D5B27-Immunohistochemistry-Paraffin-NB110-96423-img0001.jpg "Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - Staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle.")
![Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-LF3-B7-D5B27-Immunohistochemistry-NB110-96423-img0002.jpg "Immunohistochemistry: Podoplanin Antibody (LF3/B7/D5B27) [NB110-96423] - staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle.")
![Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Sarcomatoid-type malignant mesothelioma observed in the MWCNT B high-dose group. A: H & E, low magnification of tumor tissue invading the diaphragm (20×). B & C: H & E, high-power view (40×). D: High-power view of anti-vimentin immunohistochemistry showing a strongly positive result (40×). E: High-power view of anti-pan-cytokeratin immunohistochemistry showing several positive cells (40×). F: High-power view of anti-podoplanin immunohistochemistry revealing positively stained cytoplasm of many cells (40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96423_mouse-monoclonal-podoplanin-antibody-lf3-b7-d5b27-31020241528451.jpg "Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Sarcomatoid-type malignant mesothelioma observed in the MWCNT B high-dose group. A: H & E, low magnification of tumor tissue invading the diaphragm (20×). B & C: H & E, high-power view (40×). D: High-power view of anti-vimentin immunohistochemistry showing a strongly positive result (40×). E: High-power view of anti-pan-cytokeratin immunohistochemistry showing several positive cells (40×). F: High-power view of anti-podoplanin immunohistochemistry revealing positively stained cytoplasm of many cells (40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Biphasic-type malignant mesothelioma observed in the MWCNT D low-dose group. A: H & E, low magnification of tumor tissue found on the surface of the omentum (20×). B & C: H & E, high-power view (40×). D: High-power view of anti-vimentin immunohistochemistry showing a strongly positive result (40×). E: High-power view of anti-pan-cytokeratin immunohistochemistry showing positive staining of the majority of cells (40×). F: High-power view of anti-podoplanin immunohistochemistry revealing positively stained cytoplasm of many cells (40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96423_mouse-monoclonal-podoplanin-antibody-lf3-b7-d5b27-310202415371979.jpg "Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Biphasic-type malignant mesothelioma observed in the MWCNT D low-dose group. A: H & E, low magnification of tumor tissue found on the surface of the omentum (20×). B & C: H & E, high-power view (40×). D: High-power view of anti-vimentin immunohistochemistry showing a strongly positive result (40×). E: High-power view of anti-pan-cytokeratin immunohistochemistry showing positive staining of the majority of cells (40×). F: High-power view of anti-podoplanin immunohistochemistry revealing positively stained cytoplasm of many cells (40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry showing single asbestos fibers in the tissue (arrows). C: H & E, high-power view of granuloma induced by MWCNT A (low dose) nanotubes including single nanotube (arrow, 25×). D: H & E, high-power view of granuloma induced by asbestos including single fiber (arrow, 40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96423_mouse-monoclonal-podoplanin-antibody-lf3-b7-d5b27-310202415175257.jpg "Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry showing single asbestos fibers in the tissue (arrows). C: H & E, high-power view of granuloma induced by MWCNT A (low dose) nanotubes including single nanotube (arrow, 25×). D: H & E, high-power view of granuloma induced by asbestos including single fiber (arrow, 40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry showing single asbestos fibers in the tissue (arrows). C: H & E, high-power view of granuloma induced by MWCNT A (low dose) nanotubes including single nanotube (arrow, 25×). D: H & E, high-power view of granuloma induced by asbestos including single fiber (arrow, 40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96423_mouse-monoclonal-podoplanin-antibody-lf3-b7-d5b27-310202415334937.jpg "Western Blot: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Non-neoplastic histopathological findings in the abdominal cavity. A: High-power view of anti-podoplanin immunohistochemistry showing single MWCNT A (high dose) nanotubes in the tissue (arrows). B: High-power view of anti-podoplanin immunohistochemistry showing single asbestos fibers in the tissue (arrows). C: H & E, high-power view of granuloma induced by MWCNT A (low dose) nanotubes including single nanotube (arrow, 25×). D: H & E, high-power view of granuloma induced by asbestos including single fiber (arrow, 40×). Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Biphasic-type malignant mesothelioma observed in the amosite asbestos group. A: H & E, low magnification of tumor tissue found on the liver surface (10×). B: H & E, higher magnification (20×). C: H & E, high-power view (40×). D: High-power view of anti-vimentin immunohistochemistry showing a strongly positive result (40×). E: High-power view of anti-pan-cytokeratin immunohistochemistry showing positive staining of many cells (40×). F: High-power view of anti-podoplanin immunohistochemistry revealing positivity in distinct parts of the tumor. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96423_mouse-monoclonal-podoplanin-antibody-lf3-b7-d5b27-310202415291987.jpg "Immunohistochemistry-Paraffin: Podoplanin Antibody (LF3/B7/D5B27) - Azide and BSA Free [NB110-96423] - Biphasic-type malignant mesothelioma observed in the amosite asbestos group. A: H & E, low magnification of tumor tissue found on the liver surface (10×). B: H & E, higher magnification (20×). C: H & E, high-power view (40×). D: High-power view of anti-vimentin immunohistochemistry showing a strongly positive result (40×). E: High-power view of anti-pan-cytokeratin immunohistochemistry showing positive staining of many cells (40×). F: High-power view of anti-podoplanin immunohistochemistry revealing positivity in distinct parts of the tumor. Image collected & cropped by CiteAb from the following publication (//particleandfibretoxicology.biomedcentral.com/articles/10.1186/s12989-014-0059-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")