Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with VEGFR3/FLT4, another marker for lymphatic endothelial cells. It appears to be involved in lymphangioigenesis and cell migration and is regulated by the lymphatic-specific homeobox gene Prox1. Podoplanin has also been found to be expressed on a wide variety of tumors. Immunohistochemical studies with the podoplanin antibody from Schacht’s lab is a crucial player in directing the processes of cell adhesion, migration, and tube formation for lymphatic vasculature formation1.
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Immunohistochemistry-Paraffin: Podoplanin Antibody
A research group from the University of Basel relied upon the podoplanin antibody to validate their NCAM-deficient transgenic mice as a model system for endogenous tumor lymphangioigenesis and lymph node metastasis2. Findings from Steele et al used the podoplanin antibody in flow cytometry coupled with a protein-based screening strategy to identify a novel beta1 integrin-dependent mechanism of matrix metalloproteinase-10 (MMP10) in lymphatic vessel remodeling3. Recently, the podoplanin antibody allowed researchers at Wayne State University to study the effects of the small molecular inhibitor CARP-1 functional mimetics (CFMs) on malignant pleural mesothelioma (MPM)4. Related gene array studies by the same group also used the podoplanin antibody to investigate the FDA-approved compound for alcoholism treatment disulfiram (DSF) which also has other interesting properties – those of anti-tumor and chemosensitizing5. DSF appears to suppress mesothelioma growth by inducing apoptosis through NFkB- and stress activated protein kinase (SAPK)-dependent signaling pathways. Novus Biologicals offers Podoplanin reagents for your research needs including:
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