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PIM2 Antibody (OTI5D5) - Azide and BSA Free

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Western Blot: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.
Western Blot: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2.
Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of paraffin-embedded Human prostate tissue using anti-PIM2 mouse monoclonal antibody.
Flow Cytometry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PIM2 ...read more
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of HeLa cells using anti-PIM2 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of HT29 cells using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of paraffin-embedded Human Kidney tissue using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of paraffin-embedded Human liver tissue using anti-PIM2 mouse monoclonal antibody.
Immunohistochemistry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Staining of paraffin-embedded Human pancreas tissue using anti-PIM2 mouse monoclonal antibody.
Flow Cytometry: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - Analysis of Jurkat cells, using anti-PIM2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunoprecipitation: PIM2 Antibody (OTI5D5) - Azide and BSA Free [NBP2-73412] - (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
OTI5D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73412AF350
Alexa Fluor 405
NBP2-73412AF405
Alexa Fluor 488
NBP2-73412AF488
Alexa Fluor 532
NBP2-73412AF532
Alexa Fluor 594
NBP2-73412AF594
Alexa Fluor 647
NBP2-73412AF647
Alexa Fluor 700
NBP2-73412AF700
Alexa Fluor 750
NBP2-73412AF750
Allophycocyanin
NBP2-73412APC
Allophycocyanin/Cy7
NBP2-73412APCCY7
Biotin
NBP2-73412B
CoraFluor 1
NBP2-73412CL1
DyLight 350
NBP2-73412UV
DyLight 405
NBP2-73412V
DyLight 488
NBP2-73412G
DyLight 550
NBP2-73412R
DyLight 594
NBP2-73412DL594
DyLight 650
NBP2-73412C
DyLight 680
NBP2-73412FR
DyLight 755
NBP2-73412IR
FITC
NBP2-73412F
HRP
NBP2-73412H
Janelia Fluor 525
NBP2-73412JF525
Janelia Fluor 549
NBP2-73412JF549
Janelia Fluor 585
NBP2-73412JF585
Janelia Fluor 635
NBP2-73412JF635
Janelia Fluor 646
NBP2-73412JF646
Janelia Fluor 669
NBP2-73412JF669
PE
NBP2-73412PE
PE/Atto594
NBP2-73412PEATT594
PE/Cy5.5
NBP2-73412PECY55
PE/Cy7
NBP2-73412PECY7
PerCP
NBP2-73412PCP
Unconjugated
NBP2-02441
mFluor Violet 450 SE
NBP2-73412MFV450
mFluor Violet 500 SE
NBP2-73412MFV500
mFluor Violet 610 SE
NBP2-73412MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02441
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PIM2 Antibody (OTI5D5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PIM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:2000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PIM2 Antibody (OTI5D5) - Azide and BSA Free

  • EC 2.7.11.1
  • pim-2 oncogene
  • PIM2
  • pim-2h
  • proto-oncogene Pim-2 (serine threonine kinase)
  • serine/threonine protein kinase pim-2
  • serine/threonine-protein kinase pim-2

Background

PIM2 belongs to a family of cytoplasmic serine/threonine protein kinases that consists of two other members, PIM1 and PIM3. Expression of this protein is regulated by hematopoietic cytokines. PIM2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It is also highly expressed in human leukemic and lymphoma cell lines. The gene is also expressed in primary spermatocytes. The results suggest a role for PIM2 in proliferating cells as well as during meiosis. PIM2 is thought to be a mediator of p53 mediated apoptosis inhibition by interleukin 3, which supports its role in the formation of various tumours including lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PIM2