Mcl-1 Antibody

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. This antibody recognizes Mcl-1, isoforms 1 and 2. Human Mcl-1 isoform 1 is a 350 amino acid protein, GenBank no. NP_068779.1. Human Mcl-1 isoform 2 is 271 amino acid protein, GenBank no. NP_877495.1.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, WB.

Showing Publications 1 - 5 of 5.

Publications using NB100-56146	Applications	Species
Srivastava S, Gajwani P, Jousma J et al. Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism Nature communications 2023-07-19 [PMID: 37468519] (WB, Human) Details: Dilutions: 1:1000	WB	Human
Saleh T, El-Sadoni M, Alhesa A Et al. Expression of Senescence and Apoptosis Biomarkers in Synchronous Bilateral Breast Cancer: A Case Report Current oncology (Toronto, Ont.) 2021-09-30 [PMID: 34677245] (IF/IHC, Human)	IF/IHC	Human
Ohta K, Iwai K, Kasahara Y et al. Immunoblot analysis of cellular expression of Bcl-2 family proteins, Bcl-2, Bax, Bcl-X and Mcl-1, in human peripheral blood and lymphoid tissues. Int Immunol. 1995-11-01 [PMID: 8580080] (WB, Human) Details: WB: Bcl-2 (IMG-5685), Mcl-1 (IMG-5733), Bax (IMG-5682), Bcl-X (IMG-80459). 1. WB: Human PBMC, neutrophils, tonsil, spleen, thymus, Fig 1A.	WB	Human
Reynolds JE, Yang T, Qian L et al. Mcl-1, a member of the Bcl-2 family, delays apoptosis induced by c-Myc overexpression in Chinese hamster ovary cells. Cancer Res. 1994-12-15 [PMID: 7987827] (WB) Details: Mcl-1 (IMG-5733). WB: Untransfected Chinese hamster ovary 5AHSmyc cells, Bcl-2 transfected 5AHSmyc cells, and human myeloblastic ML-1 cells treated with TPA, Fig 2C.	WB
Krajewski S, Bodrug S, Krajewska M et al. Immunohistochemical analysis of Mcl-1 protein in human tissues. Differential regulation of Mcl-1 and Bcl-2 protein production suggests a unique role for Mcl-1 in control of programmed cell death in vivo. Am J Pathol. 1995-06-01 [PMID: 7778670]

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Human	06/15/2016	View Summary Application Western Blot Sample Tested Whole cell lysate from UW228 cells Species Human Lot AR03061206B-02

Array NB100-56146

• Mcl-1 Antibodies
• Mcl-1 ELISA Kits
• Mcl-1 Proteins
• Mcl-1 Proteins and Enzyme