![Western Blot: PIM2 Antibody (OTI5D5) [NBP2-02441] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Western-Blot-NBP2-02441-img0003.jpg "Western Blot: PIM2 Antibody (OTI5D5) [NBP2-02441] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | OTI5D5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.54 mg/ml |
| Immunogen | Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PIM2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 34 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.54 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for PIM2 Antibody (NBP2-02441)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2.](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Immunocytochemistry-Immunofluorescence-NBP2-02441-img0011.jpg "Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2.")
![Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human prostate tissue using anti-PIM2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Immunohistochemistry-Paraffin-NBP2-02441-img0010.jpg "Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human prostate tissue using anti-PIM2 mouse monoclonal antibody.")
![Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIM2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Flow-Cytometry-NBP2-02441-img0002.jpg "Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIM2 antibody, and then analyzed by flow cytometry.")
![Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441] - Immunofluorescent staining of HeLa cells using anti-PIM2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Immunocytochemistry-Immunofluorescence-NBP2-02441-img0004.jpg "Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441] - Immunofluorescent staining of HeLa cells using anti-PIM2 mouse monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441] - Immunofluorescent staining of HT29 cells using anti-PIM2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Immunocytochemistry-Immunofluorescence-NBP2-02441-img0005.jpg "Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441] - Immunofluorescent staining of HT29 cells using anti-PIM2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIM2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Immunohistochemistry-Paraffin-NBP2-02441-img0006.jpg "Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIM2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human Kidney tissue using anti-PIM2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Immunohistochemistry-Paraffin-NBP2-02441-img0007.jpg "Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human Kidney tissue using anti-PIM2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human liver tissue using anti-PIM2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Immunohistochemistry-Paraffin-NBP2-02441-img0008.jpg "Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human liver tissue using anti-PIM2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human pancreas tissue using anti-PIM2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Immunohistochemistry-Paraffin-NBP2-02441-img0009.jpg "Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human pancreas tissue using anti-PIM2 mouse monoclonal antibody.")
![Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441] - Analysis of Jurkat cells, using anti-PIM2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Flow-Cytometry-NBP2-02441-img0001.jpg "Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441] - Analysis of Jurkat cells, using anti-PIM2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Immunoprecipitation: PIM2 Antibody (OTI5D5) [NBP2-02441] - (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PIM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/PIM2-Antibody-OTI5D5-Immunoprecipitation-NBP2-02441-img0012.jpg "Immunoprecipitation: PIM2 Antibody (OTI5D5) [NBP2-02441] - (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PIM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.")
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