![Western Blot: PIASy Antibody [NBP1-31215] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human PIAS4-transfected 293T cells 10% SDS-PAGE PIASy antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PIASy-Antibody-Western-Blot-NBP1-31215-img0008.jpg "Western Blot: PIASy Antibody [NBP1-31215] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human PIAS4-transfected 293T cells 10% SDS-PAGE PIASy antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Bv, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PIASy. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Chimpanzee (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PIAS4 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publications using NBP1-31215 | Applications | Species |
|---|---|---|
| Agboola AO, Ebili HO, Iyawe VO et al. Clinicopathological and molecular characteristics of Ku 70/80 expression in Nigerian breast cancer and its potential therapeutic implications Pathology - Research and Practice 2017-01-01 [PMID: 27914769] (Immunohistochemistry, Immunohistochemistry-Paraffin) | Immunohistochemistry, Immunohistochemistry-Paraffin | |
| Alshareeda AT, Negm OH, Green AR et al. SUMOylation proteins in breast cancer. Breast Cancer Res. Treat. 2014-03-03 [PMID: 24584753] (IHC-P, Human) | IHC-P | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for PIASy Antibody (NBP1-31215)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PIAS4 |
![Immunocytochemistry/Immunofluorescence: PIASy Antibody [NBP1-31215] - Paraformaldehyde-fixed HeLa, using PIASy antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/PIASy-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31215-img0003.jpg "Immunocytochemistry/Immunofluorescence: PIASy Antibody [NBP1-31215] - Paraformaldehyde-fixed HeLa, using PIASy antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: PIASy Antibody [NBP1-31215] - Mouse colon. PIASy antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PIASy-Antibody-Immunohistochemistry-NBP1-31215-img0006.jpg "Immunohistochemistry-Paraffin: PIASy Antibody [NBP1-31215] - Mouse colon. PIASy antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: PIASy Antibody [NBP1-31215] - Paraffin-embedded DLD1 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PIASy-Antibody-Immunohistochemistry-Paraffin-NBP1-31215-img0004.jpg "Immunohistochemistry-Paraffin: PIASy Antibody [NBP1-31215] - Paraffin-embedded DLD1 Xenograft, using antibody at 1:100 dilution.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")