![Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NBP1-76741-img0006.jpg "Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human PERP. The immunogen is located within the last 50 amino acids of PERP. Amino Acid Sequence: EDDLLGNAKPRYFYTSA |
| Specificity | Human PERP has one isoform (193aa, 21kD). Mouse PERP has one isoform (193aa, 21.6kD) and Rat PERP also has one isoform (193aa, 21.6kD). NBP1-76741 can detect human, mouse and rat. |
| Predicted Species | Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PERP |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for PERP Antibody (NBP1-76741)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PERP Antibody [NBP1-76741] - Immunocytochemical analysis of PERP in formalin-fixed A431 cells with PERP antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76741-img0002.jpg "Immunocytochemistry/Immunofluorescence: PERP Antibody [NBP1-76741] - Immunocytochemical analysis of PERP in formalin-fixed A431 cells with PERP antibody at 10 ug/ml.")
![Western Blot: PERP Antibody [NBP1-76741] - Independent Antibody Validation via Protein Expression Profile in Human Cell Lines. Loading: 15 ug lysates per lane. Antibodies: PERP NBP1-76741 (1ug/ml), PERP antibody from Company B (2 ug/ml), beta-actin NBP1-76692 (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NBP1-76741-img0004.jpg "Western Blot: PERP Antibody [NBP1-76741] - Independent Antibody Validation via Protein Expression Profile in Human Cell Lines. Loading: 15 ug lysates per lane. Antibodies: PERP NBP1-76741 (1ug/ml), PERP antibody from Company B (2 ug/ml), beta-actin NBP1-76692 (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NBP1-76741-img0005.jpg "Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/ Immunofluorescence: PERP Antibody - BSA Free [NBP1-76741] - Figure 4 Immunocytochemistry Validation of PERP in A431 CellsImmunocytochemical analysis of A431 cells using anti-PEPR antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76741_rabbit-polyclonal-perp-antibody-271120241923644.jpg "Immunocytochemistry/ Immunofluorescence: PERP Antibody - BSA Free [NBP1-76741] - Figure 4 Immunocytochemistry Validation of PERP in A431 CellsImmunocytochemical analysis of A431 cells using anti-PEPR antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunocytochemistry/ Immunofluorescence: PERP Antibody - BSA Free [NBP1-76741] - Figure 4 Immunocytochemistry Validation of PERP in A431 CellsImmunocytochemical analysis of A431 cells using anti-PEPR antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76741_rabbit-polyclonal-perp-antibody-2711202419172298.jpg "Immunocytochemistry/ Immunofluorescence: PERP Antibody - BSA Free [NBP1-76741] - Figure 4 Immunocytochemistry Validation of PERP in A431 CellsImmunocytochemical analysis of A431 cells using anti-PEPR antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")