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![Western Blot: PERP Antibody (1O3L5) [NBP3-16617] - Western blot analysis of extracts of various cell lines, using PERP Rabbit mAb (NBP3-16617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Western-Blot-NBP3-16617-img0005.jpg "Western Blot: PERP Antibody (1O3L5) [NBP3-16617] - Western blot analysis of extracts of various cell lines, using PERP Rabbit mAb (NBP3-16617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: PERP Antibody (1O3L5) [NBP3-16617] - Immunofluorescence analysis of mouse skin cells using PERP Rabbit mAb (NBP3-16617) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Immunohistochemistry-NBP3-16617-img0004.jpg)
![Western Blot: PERP Antibody (1O3L5) [NBP3-16617] - Western blot analysis of extracts of various cell lines, using PERP Rabbit mAb (NBP3-16617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Western-Blot-NBP3-16617-img0006.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody (1O3L5) [NBP3-16617] - Immunohistochemistry of paraffin-embedded rat skin using PERP Rabbit mAb (NBP3-16617) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Immunohistochemistry-Paraffin-NBP3-16617-img0001.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody (1O3L5) [NBP3-16617] - Immunohistochemistry of paraffin-embedded mouse skin using PERP Rabbit mAb (NBP3-16617) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Immunohistochemistry-Paraffin-NBP3-16617-img0002.jpg)
![Immunohistochemistry: PERP Antibody (1O3L5) [NBP3-16617] - Immunofluorescence analysis of human skin cells using PERP Rabbit mAb (NBP3-16617) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Immunohistochemistry-NBP3-16617-img0003.jpg)
![Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NBP1-76741-img0006.jpg "Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/Immunofluorescence: PERP Antibody [NBP1-76741] - Immunocytochemical analysis of PERP in formalin-fixed A431 cells with PERP antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76741-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PERP Antibody [NBP1-76741] - Independent Antibody Validation via Protein Expression Profile in Human Cell Lines. Loading: 15 ug lysates per lane. Antibodies: PERP NBP1-76741 (1ug/ml), PERP antibody from Company B (2 ug/ml), beta-actin NBP1-76692 (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NBP1-76741-img0004.jpg)
![Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NBP1-76741-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: PERP Antibody - BSA Free [NBP1-76741] - Figure 4 Immunocytochemistry Validation of PERP in A431 CellsImmunocytochemical analysis of A431 cells using anti-PEPR antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76741_rabbit-polyclonal-perp-antibody-271120241923644.jpg)
![Immunocytochemistry/ Immunofluorescence: PERP Antibody - BSA Free [NBP1-76741] - Figure 4 Immunocytochemistry Validation of PERP in A431 CellsImmunocytochemical analysis of A431 cells using anti-PEPR antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76741_rabbit-polyclonal-perp-antibody-2711202419172298.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human esophagus shows very weak membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0010.jpg "Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human esophagus shows very weak membranous positivity in squamous epithelial cells.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Analysis in human skin and skeletal muscle tissues. Corresponding PERP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0006.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human skin shows moderate to strong membranous positivity in stratum corneum.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0007.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human lymph node shows no positivity as expected.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0008.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human endometrium shows no positivity as expected.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0009.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody [NBP1-85173] - Staining of human skeletal muscle shows no positivity as expected.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NBP1-85173-img0011.jpg)
![Western Blot: PERP Antibody [NB100-2912] - (1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NB100-2912-img0002.jpg "Western Blot: PERP Antibody [NB100-2912] - (1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PERP Antibody [NB100-2912] - Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PERP-Antibody-Immunohistochemistry-Paraffin-NB100-2912-img0003.jpg)
![Western Blot: PERP Antibody [NBP1-62434] - PANC1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PERP-Antibody-Western-Blot-NBP1-62434-img0002.jpg "Western Blot: PERP Antibody [NBP1-62434] - PANC1 cell lysate, concentration 0.2-1 ug/ml.")
![SDS-Page: Recombinant Human PERP Protein [H00064065-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-PERP-Protein-SDS-Page-H00064065-P01-img0002.jpg "SDS-Page: Recombinant Human PERP Protein [H00064065-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: PERP Overexpression Lysate (Adult Normal) [NBP2-06463] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PERP.](http://images.novusbio.com/fullsize/PERP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06463-img0001.jpg "Western Blot: PERP Overexpression Lysate (Adult Normal) [NBP2-06463] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PERP.")
![Western Blot: PERP Peptide [NBP1-76741PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C](http://images.novusbio.com/fullsize/PERP-Peptide-Western-Blot-NBP1-76741PEP-img0001.jpg "Western Blot: PERP Peptide [NBP1-76741PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C")