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Perilipin-3/TIP47 Antibody (779119)

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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
779119
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Perilipin-3/TIP47 Antibody (779119) Summary

Immunogen
E. coli-derived recombinant human Perilipin‑3
Met1-Lys434
Accession # O60664
Specificity
Detects human Perilipin‑3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Perilipin-2 or rhPerilipin-5 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PLIN3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Perilipin-3/TIP47 Antibody (779119)

  • Cargo selection protein TIP47
  • M6PRBP1
  • M6PRBP1MGC2012
  • Mannose-6-phosphate receptor-binding protein 1
  • perilipin 3,47 kDa MPR-binding protein
  • Perilipin3
  • Perilipin-3
  • Placental protein 17,47 kDa mannose 6-phosphate receptor-binding protein
  • PLIN3
  • PP17
  • PP17MGC11117
  • tail-interacting protein, 47 kD
  • TIP47
  • TIP47mannose-6-phosphate receptor binding protein 1

Background

M6PRBP1 (mannose 6-phosphate receptor binding protein), gene name PLIN3 (Perilipin-3), also called TIP47 (tail interacting protein of 47 kDa) or PP17 (placental protein 17), is a 47 kDa intracellular transport protein that belongs to the PAT (Perilipin/Adipophilin/TIP47) family of molecules. The 434 amino acid (aa) human Perilipin‑3, which shares approximately 75% aa sequence identity with mouse and rat Perilipin-3, contains a four-helix bundle thought to interact with membranes, and may be phosphorylated and acetylated. A 251 aa form (isoform A) has an alternate start site at aa 184 of the 434 aa form (isoform B). Perilipin-3 is normally found predominantly in the cytoplasm. It is required for uptake of mannose-6 phosphate receptors into endosomes. In the presence of excess lipids, such as in macrophage-derived foam cells, Perilipin-3 may transfer free fatty acids to lipid droplets, promoting their growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PLIN3
Uniprot