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![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Perilipin-3/TIP47 Antibody (NB110-40764) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40764-img0007.jpg "Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Perilipin-3/TIP47 Antibody (NB110-40764) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: Perilipin-3/TIP47 Antibody [NB110-40764] - Detection of TIP47 in 3T3 L1 lysate.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Western-Blot-NB110-40764-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Perilipin-3/TIP47 Antibody conjugated to DyLight 550 (NB110-40764R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40764-img0008.jpg)
![Western Blot: Perilipin-3/TIP47 Antibody [NB110-40764] - Changes in protein and gene expression relating to lipolysis with a single bout of endurance exercise in human skeletal muscle. Representative blots of PLIN3, ATGL, ARF1, ARFRP1 and loading control GAPDH. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0091675), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Western-Blot-NB110-40764-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40764] - TIP47 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40764-img0005.jpg)
![Western Blot: Perilipin-3/TIP47 Antibody [NBP1-87871] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Western-Blot-NBP1-87871-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Perilipin-3/TIP47 Antibody [NBP1-87871] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human cell line A-431 shows positivity in vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87871-img0008.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0013.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human cervix shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0010.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0011.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP1-87871] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP1-87871-img0012.jpg)
![Western Blot: Perilipin-3/TIP47 Antibody [NB110-40765] - Detection of TIP47 in 3T3 L1 lysate.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Western-Blot-NB110-40765-img0003.jpg "Western Blot: Perilipin-3/TIP47 Antibody [NB110-40765] - Detection of TIP47 in 3T3 L1 lysate.")
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40765] - TIP47 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40765-img0004.jpg)
![Simple Western: Perilipin-3/TIP47 Antibody [NB110-40765] - Simple Western lane view shows a specific band for TIP47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Simple-Western-NB110-40765-img0005.jpg)
![Western Blot: Perilipin-3/TIP47 Antibody [NBP2-49485] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Western-Blot-NBP2-49485-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Perilipin-3/TIP47 Antibody [NBP2-49485] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP2-49485] - Analysis of human cell line A-431 shows positivity in vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49485-img0001.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP2-49485] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP2-49485-img0008.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP2-49485] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP2-49485-img0005.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP2-49485] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP2-49485-img0006.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP2-49485] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP2-49485-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP2-97277] - Immunofluorescence staining of Perilipin-3/TIP47 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Perilipin-3/TIP47 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97277-img0001.jpg "Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP2-97277] - Immunofluorescence staining of Perilipin-3/TIP47 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Perilipin-3/TIP47 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP2-97277] - Immunochemical staining of human PLIN3 in human small intestine with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP2-97277-img0003.jpg)
![Immunohistochemistry-Paraffin: Perilipin-3/TIP47 Antibody [NBP2-97277] - Immunochemical staining of human Perilipin-3/TIP47 in human adrenal gland with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunohistochemistry-Paraffin-NBP2-97277-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP1-06049] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and lipid droplet staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06049-img0001.jpg "Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NBP1-06049] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and lipid droplet staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![SDS-Page: Recombinant Human Perilipin-3/TIP47 Protein [H00010226-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Perilipin-3-TIP47-Protein-SDS-Page-H00010226-P01-img0002.jpg "SDS-Page: Recombinant Human Perilipin-3/TIP47 Protein [H00010226-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: TIP47 Overexpression Lysate (Adult Normal) [NBL1-12781] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIP47.](http://images.novusbio.com/fullsize/TIP47-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12781-img0002.jpg "Western Blot: TIP47 Overexpression Lysate (Adult Normal) [NBL1-12781] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIP47.")