Perilipin-2/ADFP Antibody - BSA Free

Perilipin-2/Plin-2, also known as adipose differentiation-related protein (ADFP), ADRP, and adipophilin, is a member of the perilipin family of lipid droplet (LD) proteins that functions in lipid accumulation, lipid storage, and LD formation (1,2). Perilipin-2 is ubiquitously expressed and is the major LD protein of the liver (1-3). Human perilipin-2 is 437 amino acids (aa) in length with a theoretical molecular weight of 48 kDa (1,4). Human and mouse perilipin-2 share 81.8% sequence identity. One defining structural feature of perilipin-2 and other LD-related proteins is formation of amphipathic alpha helices (1,3,5). The perilipin-2 protein contains a PAT (perilipin A, ADRP, TIP47) domain, a 11-mer repeat motif, and a 4-helix bundle (1,5). The PAT domain has a role in triglyceride (TG) stabilization, the 11-mer repeat motif functions in cytoplasmic LD binding, and the 4-helix bundle facilitates membrane binding (5). Unlike LDs coated with other perilipin family members (e.g., perilipin-1 and perilipin-5), LDs coated with perilipin-2 are more permissive to lipolysis and protective of lipids and TGs (1-3). Studies have revealed that perilipin-2 knockout (KO) in mice is associated with resistance to fatty liver disease and diet-induced obesity (1). On the other hand, overexpression of perilipin-2 causes increased lipid and TG accumulation, which is a feature of many metabolic, cardiovascular, and age-related diseases (2). Additional research has found higher levels of perilipin-2 is associated with certain cancers such as colorectal cancer and renal carcinomas, and it may be a useful biomarker for detection (2). Though more research is needed, it is possible that inhibition or blocking of perilipin-2 may be a key strategy to combatting several pathologies (2).

References

1. Itabe H, Yamaguchi T, Nimura S, Sasabe N. Perilipins: a diversity of intracellular lipid droplet proteins. Lipids Health Dis. 2017;16(1):83. https://doi.org/10.1186/s12944-017-0473-y

2. Conte M, Franceschi C, Sandri M, Salvioli S. Perilipin 2 and Age-Related Metabolic Diseases: A New Perspective. Trends Endocrinol Metab. 2016;27(12):893-903. https://doi.org/10.1016/j.tem.2016.09.001

3. Sztalryd C, Brasaemle DL. The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis. Biochim Biophys Acta Mol Cell Biol Lipids. 2017;1862(10 Pt B):1221-1232. https://doi.org/10.1016/j.bbalip.2017.07.009

4. Uniprot (Q99541)

5. Chong BM, Reigan P, Mayle-Combs KD, Orlicky DJ, McManaman JL. Determinants of adipophilin function in milk lipid formation and secretion. Trends Endocrinol Metab. 2011;22(6):211-217. https://10.1016/j.tem.2011.04.003

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Plant.

We have publications tested in 9 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, Immunocytochemistry/ Immunofluorescence, SDS-Page, WB, Western Blot.

Showing Publications 1 - 10 of 65. Show All 65 Publications. Collapse Publications.

Publications using NB110-40877	Applications	Species
Kloosterman DJ, Erbani J, Boon M et Al. Macrophage-mediated myelin recycling fuels brain cancer malignancy Cell 2024-09-19 [PMID: 39137777]
Ibayashi M, Tatsumi T, Tsukamoto S. Perilipin2 depletion causes lipid droplet enlargement in the ovarian corpus luteum in mice The Journal of Reproduction and Development 2024-07-15 [PMID: 39010158]
Junichiro MITSUI, Megumi IBAYASHI, Ryutaro AIZAWA, Tomonori ISHIKAWA, Naoyuki MIYASAKA, Satoshi TSUKAMOTO Lipid droplets synthesized during luteinization are degraded after pregnancy The Journal of Reproduction and Development 2024-02-04 [PMID: 38311402]
Renier TJ, Paetz OR, Paal MC et al. Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins microPublication biology 2023-11-03 [PMID: 38021172] (SDS-Page, Mouse)	SDS-Page	Mouse
Kothari V, Savard C, Tang J et al. sTREM2 is a plasma biomarker for human NASH and promotes hepatocyte lipid accumulation Hepatology communications 2023-11-01 [PMID: 37820278] (ICC/IF, Mouse)	ICC/IF	Mouse
Swathy Krishna, Kenneth G. Echevarria, Carter H. Reed, Hyeyoon Eo, Michelle Wintzinger, Mattia Quattrocelli, Rudy J. Valentine, Joshua T. Selsby A fat- and sucrose-enriched diet causes metabolic alterations in mdx mice American Journal of Physiology - Regulatory, Integrative and Comparative Physiology 2023-12-01 [PMID: 37811713]
Wieland EB, Kempen LJAP, Lu C et al. Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial context STAR protocols 2023-09-23 [PMID: 37742177] (IHC-Fr) Details: Alexa Fluor 488 conjugation used. Diltion 1:29	IHC-Fr
Ibayashi M, Aizawa R, Mitsui J, Tsukamoto S Lipid droplet synthesis is associated with angiogenesis in mouse ovarian follicles Biology of reproduction 2022-12-28 [PMID: 36579469]
Yu S, Wang L, Che D Et al. Targeting CRABP-II Overcomes Pancreatic Cancer Drug Resistance By Reversing Lipid Raft Cholesterol Accumulation and AKT Survival Signaling Research Square Sep 30 2021 (IHC-P, Human)	IHC-P	Human
Choi YJ, Yun SH, Yu J et al. Chaperone-mediated autophagy dysregulation during aging impairs hepatic fatty acid oxidation via accumulation of NCoR1 Molecular metabolism 2023-07-29 [PMID: 37524243] (WB, Mouse)	WB	Mouse
Androvic P, Schifferer M, Perez Anderson K et al. Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury Nature communications 2023-07-11 [PMID: 37433806] (IHC, Mouse)	IHC	Mouse
Pratelli G, Di Liberto D, Carlisi D et al. Hypertrophy and ER Stress Induced by Palmitate Are Counteracted by Mango Peel and Seed Extracts in 3T3-L1 Adipocytes International journal of molecular sciences 2023-03-12 [PMID: 36982490] (WB, Plant)	WB	Plant
Mi?i? B, Djordjevic A, Veli?kovi? N et al. AMPK Activation as a Protective Mechanism to Restrain Oxidative Stress in the Insulin-Resistant State in Skeletal Muscle of Rat Model of PCOS Subjected to Postnatal Overfeeding Biomedicines 2023-05-30 [PMID: 37371678]
Wang N, Wang M, Jeevaratnam S et al. Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination Molecular neurodegeneration 2022-11-23 [PMID: 36419137] (IF/IHC, Mouse)	IF/IHC	Mouse
Lin M, Ku AT, Dong J et al. STAT5 confers lactogenic properties in breast tumorigenesis and restricts metastatic potential Oncogene 2022-10-19 [PMID: 36261627] (IF/IHC, Mouse)	IF/IHC	Mouse
Fraunhoffer NA, Abuelafia AM, Bigonnet M et al. Multi-omics data integration and modeling unravels new mechanisms for pancreatic cancer and improves prognostic prediction NPJ precision oncology 2022-08-17 [PMID: 35978026] (IHC-P, Human) Details: Dilutions: 1:100	IHC-P	Human
Liu Q, Xiao JJ, Wang S et al. Paraoxonase 1 Ameliorates Renal Lipotoxicity by Activating Lipophagy and Inhibiting Pyroptosis The American journal of pathology 2022-08-10 [PMID: 35963464]
Goossens P, Lu C, Cao J et al. Integrating multiplex immunofluorescent and mass spectrometry imaging to map myeloid heterogeneity in its metabolic and cellular context Cell metabolism 2022-07-12 [PMID: 35858629] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Wang Z, Wu Q, Wang H et al. Diosgenin protects against podocyte injury in early phase of diabetic nephropathy through regulating SIRT6 Phytomedicine : international journal of phytotherapy and phytopharmacology 2022-06-13 [PMID: 35728388] (IF/IHC, Mouse)	IF/IHC	Mouse
Antony R, Aby K, Gao H et al. UCHL1 Regulates Lipid and Perilipin 2 Level in Skeletal Muscle Frontiers in physiology 2022-04-07 [PMID: 35464088] (WB, Mouse)	WB	Mouse
Asif S, Kim RY, Fatica T et al. Hmgcs2-mediated ketogenesis modulates high-fat diet-induced hepatosteatosis Molecular metabolism 2022-04-12 [PMID: 35421611] (IHC-P, Mouse)	IHC-P	Mouse
Lee W, Kim HY, Choi YJ et al. SNX10-mediated degradation of LAMP2A by NSAIDs inhibits chaperone-mediated autophagy and induces hepatic lipid accumulation Theranostics 2022-02-21 [PMID: 35265214] (WB, ICC/IF, Human, Mouse)	WB, ICC/IF	Human, Mouse
Yu S, Wang L, Che D et al. Targeting CRABP-II overcomes pancreatic cancer drug resistance by reversing lipid raft cholesterol accumulation and AKT survival signaling Journal of experimental & clinical cancer research : CR 2022-03-08 [PMID: 35260193] (IF/IHC, Human)	IF/IHC	Human
Lu Y, Zhou T, Xu C et al. Occludin is a target of Src kinase and promotes lipid secretion by binding to BTN1a1 and XOR PLoS biology 2022-01-01 [PMID: 35041644] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Swinkels D, Das Y, Kocherlakota S et al. Cell Type-Selective Loss of Peroxisomal beta-Oxidation Impairs Bipolar Cell but Not Photoreceptor Survival in the Retina Cells 2022-01-04 [PMID: 35011723] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Sorg O, Nocera T, Fontao F Et al. Lipid Droplets Proteins in Acne Skin. A sound target for the maintenance of low comedogenic sebum and acne-prone skin health. JID Innov 2021-12-15 [PMID: 34909752]
Liu H, Wang C, Chang Z Et al. Losartan Attenuates Insulin Resistance and Regulates Browning Phenomenon of White Adipose Tissue in ob/ob Mice Curr Issues Mol Biol 2021-12-10 [PMID: 34889901]
Rajesh Y, Reghupaty SC, Mendoza RG Et al. Dissecting the Balance Between Metabolic and Oncogenic Functions of Astrocyte-Elevated Gene-1/Metadherin Hepatology communications 2021-11-05 [PMID: 34741448] (IF/IHC, Mouse)	IF/IHC	Mouse
Chen Q, Fang W, Cui K et al. Endoplasmic reticulum stress induces hepatic steatosis by transcriptional upregulating lipid droplet protein perilipin2 FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-10-01 [PMID: 34547130]
Xu S, Lee E, Sun Z et al. Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPAR alpha Journal of immunology research 2021-09-09 [PMID: 34541006] (WB, Mouse)	WB	Mouse
Casado ME, Huerta L, Marcos-DIaz A Et al. Hormone-sensitive lipase deficiency affects the expression of SR-BI, LDLr, and ABCA1 receptors/transporters involved in cellular cholesterol uptake and efflux and disturbs fertility in mouse testis Biochimica et biophysica acta. Molecular and cell biology of lipids 2021-08-27 [PMID: 34461308]
Wang CH, Liu HM, Chang ZY et al. Losartan Prevents Hepatic Steatosis and Macrophage Polarization by Inhibiting HIF-1? in a Murine Model of NAFLD International Journal of Molecular Sciences 2021-07-22 [PMID: 34360607]
MOROKI T, MATSUO S, HATAKEYAMA H et al. Databases for technical aspects of immunohistochemistry-2021 update Journal of Toxicologic Pathology 2021-02-24 [PMID: 33976473]
Qi G, Mi Y, Shi X et al. ApoE4 Impairs Neuron-Astrocyte Coupling of Fatty Acid Metabolism Cell 2021-01-05 [PMID: 33406436] (Human)		Human
Lee LL, Khakoo AY, Chintalgattu V Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease FEBS Open Bio 2020-11-01 [PMID: 33135334] (WB, Mouse) Details: C57/BL6J mouse coronary smooth muscle cells underwent Western blot analysis.	WB	Mouse
Talbott HA, Plewes MR, Krause C et al. Formation and characterization of lipid droplets of the bovine corpus luteum Sci Rep 2020-07-09 [PMID: 32647143] (WB, Bovine)	WB	Bovine
Chavanet A, Hill KR, Jim�nez-Andrade Y et al. Intracellular signaling modules linking DNA damage to secretome changes in senescent melanoma cells Melanoma Research 2020-08-01 [PMID: 32628430] (Western Blot, Immunocytochemistry/ Immunofluorescence)	Western Blot, Immunocytochemistry/ Immunofluorescence
Plewes MR, Krause C, Talbott HA et al. Trafficking of cholesterol from lipid droplets to mitochondria in bovine luteal cells: Acute control of progesterone synthesis The FASEB Journal 2020-08-01 [PMID: 32614098] (Western Blot)	Western Blot
Mayagoitia K, Shin SD, Rubini M et al. Short-term exposure to dietary cholesterol is associated with downregulation of interleukin-15, reduced thigmotaxis and memory impairment in mice Behav. Brain Res. 2020-06-22 [PMID: 32585301] (IF/IHC, Mouse)	IF/IHC	Mouse
Chen L, Ma WL, Cheng WC et al. Targeting lipid droplet lysophosphatidylcholine for cisplatin chemotherapy J. Cell. Mol. Med. 2020-06-16 [PMID: 32543783] (WB, Human)	WB	Human
Milutinovic DV, Brkljacic J, Teofilovic A et al. Chronic Stress Potentiates High Fructose-Induced Lipogenesis in Rat Liver and Kidney Mol Nutr Food Res 2020-05-07 [PMID: 32379936] (Rat)		Rat
TaIb B, Aboussalah AM, Moniruzzaman M et al. Lipid accumulation and oxidation in glioblastoma multiforme Sci Rep 2019-12-20 [PMID: 31863022] (WB, Human)	WB	Human
Helsley RN, Varadharajan V, Brown AL et al. Obesity-linked suppression of membrane-bound O-acyltransferase 7 (MBOAT7) drives non-alcoholic fatty liver disease Elife 2019-10-17 [PMID: 31621579] (Mouse)		Mouse
Asimakopoulou A, Vucur M, Luedde T et al. Perilipin 5 and Lipocalin 2 Expression in Hepatocellular Carcinoma Cancers (Basel) 2019-03-19 [PMID: 30893876] (WB, Human, Mouse)	WB	Human, Mouse
Farmer BC, Kluemper J, Johnson LA. Apolipoprotein E4 Alters Astrocyte Fatty Acid Metabolism and Lipid Droplet Formation Cells 2019-02-20 [PMID: 30791549] (WB)	WB
Chali F, Milior G, Marty S et al. Lipid markers and related transcripts during excitotoxic neurodegeneration in kainate-treated mice Eur. J. Neurosci. 2019-02-14 [PMID: 30767299] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhang P, Csaki LS, Ronquillo E et al. Lipin 2/3 phosphatidic acid phosphatases maintain phospholipid homeostasis to regulate chylomicron synthesis J. Clin. Invest. 2019-01-02 [PMID: 30507612] (WB, Human)	WB	Human
Larsen Steen, Vigelso Andreas, Dandanell Sune et al. Simvastatin-Induced Insulin Resistance May Be Linked to Decreased Lipid Uptake and Lipid Synthesis in Human Skeletal Muscle: the LIFESTAT Study. J Diabetes Res. 2018-09-12 [PMID: 30276217] (WB, Human)	WB	Human
Li Z, Rasmussen ML, Li J et al. Periodized low protein-high carbohydrate diet confers potent, but transient, metabolic improvements. Mol Metab 2018-08-28 [PMID: 30193785] (WB, Mouse)	WB	Mouse
Li Z, Rasmussen M L et al. Low- and high-protein diets do not alter ex vivo insulin action in skeletal muscle. Physiol Rep 2018-01-07 [PMID: 29998629] (WB, Mouse)	WB	Mouse
Madushani Herath KHIN, Cho J, Kim A et al. Phenolic acid and flavonoid-rich fraction of Sasa quelpaertensis Nakai leaves prevent alcohol induced fatty liver through AMPK activation J Ethnopharmacol. [PMID: 29906537] (IF/IHC, Mouse)	IF/IHC	Mouse
Blossom SJ, Fernandes L, Bai S et al. Opposing actions of developmental trichloroethylene and high-fat-diet co-exposure on markers of lipogenesis and inflammation in autoimmune-prone mice. Toxicol. Sci. 2018-04-12 [PMID: 29669109] (IF/IHC, Mouse)	IF/IHC	Mouse
Clemente C, Rius C, Alonso-Herranz L et al. MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis. Nat Commun. 2018-03-02 [PMID: 29500407] (IHC-P, Mouse)	IHC-P	Mouse
Covington JD, Johannsen DL, Coen PM et al. Intramyocellular Lipid Droplet Size Rather Than Total Lipid Content is Related to Insulin Sensitivity After 8 Weeks of Overfeeding Obesity (Silver Spring). 2017-12-01 [PMID: 29071793] (Human)		Human
Kim SE, Lee JH, Kim TG et al. Fat Graft Survival After Recipient Site Pretreatment With Fractional Carbon Dioxide Laser Ann Plast Surg. 2017-12-01 [PMID: 29053516] (Rat)		Rat
Li J, Mihalcioiu M, Li L et al. Vitamin D prevents lipid accumulation in murine muscle through regulation of PPARg and perilipin-2 expression. J. Steroid Biochem. Mol. Biol. 2017-10-14 [PMID: 29037825] (Mouse)		Mouse
Rendina-Ruedy E, Guntur AR, Rosen CJ et al. Intracellular lipid droplets support osteoblast function Adipocyte. 2017-07-19 [PMID: 28792783] (WB, Mouse)	WB	Mouse
Vigelso A, Gram M, Wiuff C et al. Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men. Eur. J. Appl. Physiol. 2015-12-01 [PMID: 26626913] (WB, Human)	WB	Human
Nishikawa K, Iwaya K, Kinoshita M et al. Resveratrol increases CD68(+) Kupffer cells co-localized with adipose differentiation-related protein (ADFP) and ameliorates high-fat-diet-induced fatty liver in mice Mol Nutr Food Res. 2015-02-12 [PMID: 25677089] (WB, Mouse) Details: Perilipin-2/ADFP antibody (0.5 ug/ml) used for WB on mouse liver extract (Fig. 4).	WB	Mouse
Bindesboll C, Berg O, Arntsen B et al. Fatty acids regulate perilipin5 in muscle by activating PPARdelta. J Lipid Res 2013-07-01 [PMID: 23606724]
Lige B, Sampels V, Coppens I. Characterization of a second sterol-esterifying enzyme in Toxoplasma highlights the importance of cholesterol storage pathways for the parasite. Mol Microbiol 2013-02-03 [PMID: 23374239] (ICC/IF, Mouse)	ICC/IF	Mouse
Chikka MR, McCabe DD, Tyra HM, Rutkowski DT. C/EBP Homologous Protein (CHOP) Contributes to Suppression of Metabolic Genes During ER Stress in the Liver. J Biol Chem 2012-12-31 [PMID: 23281479] (IHC-P, Mouse)	IHC-P	Mouse
Son S-H, Goo Y-H, Chang BH, Paul A. Perilipin 2 (PLIN2)-Deficiency Does Not Increase Cholesterol-Induced Toxicity in Macrophages PLoS ONE 2012-01-01 [PMID: 22427949] (WB, Mouse)	WB	Mouse
Wohlers LM, Jackson KC, Spangenburg EE. Lipolytic signaling in response to acute exercise is altered in female mice following ovariectomy. J Cell Biochem. 2011-08-03 [PMID: 21815195]
Show All 65 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Natasa Velickovic	WB	Rat	06/16/2020	View Summary Application Western Blot Sample Tested Liver whole cell extract,Liver whole tissue homogenate, Sample Amount: 50ug Species Rat Lot F-3 Comments Comments Concentration 1:1000, over night Secondary antibody: 1: 20000

Array NB110-40877

• Perilipin-2/ADFP Antibodies
• Perilipin-2/ADFP ELISA Kits
• Perilipin-2/ADFP Lysate
• Perilipin-2/ADFP Proteins