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CD-M6PR Antibody

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD-M6PR Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD-M6PR Lumenal Domain at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD-M6PR Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF5320
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5320-SP
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CD-M6PR Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD-M6PR Lumenal Domain
Thr27-His185
Accession # P20645
Specificity
Detects human CD-M6PR Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross‑reactivity with recombinant mouse CD-M6PR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
M6PR
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF5320 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD-M6PR Antibody

  • 000 Man6PR
  • 46 kDa mannose 6-phosphate receptor
  • 46-kDa mannose 6-phosphate receptor
  • cation-dependent mannose-6-phosphate receptor
  • CD Man-6-P receptor
  • CDM6PR
  • CD-M6PR
  • CD-MPR
  • FLJ32994
  • M6PR
  • mannose-6-phosphate receptor (cation dependent)
  • MPR 46
  • MPR46
  • MPRD
  • Mr 46
  • SMPR

Background

The CD-M6PR (cation-dependent mannose 6-phosphate receptor; also CD-MPR) is a 44‑46 kDa member of the P-type lectin family of proteins. It is ubiquitously expressed, found internally, and transports newly formed mannose 6-phosphate bearing acid hydrolases from the secretory pathway to acidified lysosomes. Mature human CD-M6PR is a type I transmembrane glycoprotein that is 251 amino acids (aa) in length. It functions as a homodimer, and contains a 159 aa luminal region (aa 27‑185) plus a 67 aa cytoplasmic domain. The aa residues Gln66, Arg111, Glu133 and Tyr143 recognize carbohydrate; Asp103 interacts with divalent cation (Mn2+), increasing ligand affinity and receptor oligomerization. Over aa 27‑184, human CD-M6PR shares 90% aa identity with mouse CD-M6PR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD-M6PR Antibody (AF5320)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol M6PR
Uniprot