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PDGF R beta Antibody (OTI1E8) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73304AF350
Alexa Fluor 405
NBP2-73304AF405
Alexa Fluor 488
NBP2-73304AF488
Alexa Fluor 532
NBP2-73304AF532
Alexa Fluor 594
NBP2-73304AF594
Alexa Fluor 647
NBP2-73304AF647
Alexa Fluor 700
NBP2-73304AF700
Alexa Fluor 750
NBP2-73304AF750
Allophycocyanin
NBP2-73304APC
Biotin
NBP2-73304B
CoraFluor 1
NBP2-73304CL1
DyLight 350
NBP2-73304UV
DyLight 405
NBP2-73304V
DyLight 488
NBP2-73304G
DyLight 550
NBP2-73304R
DyLight 594
NBP2-73304DL594
DyLight 650
NBP2-73304C
DyLight 680
NBP2-73304FR
DyLight 755
NBP2-73304IR
FITC
NBP2-73304F
HRP
NBP2-73304H
Janelia Fluor 525
NBP2-73304JF525
Janelia Fluor 646
NBP2-73304JF646
Janelia Fluor 669
NBP2-73304JF669
PE
NBP2-73304PE
PerCP
NBP2-73304PCP
Unconjugated
NBP2-46361
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46361
Azide and BSA Free
NBP2-73304
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PDGF R beta Antibody (OTI1E8) [Janelia Fluor® 549] Summary

Immunogen
Full length recombinant protein of human PDGF R beta (NP_002600) produced in HEK293T cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDGFRB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PDGF R beta Antibody (OTI1E8) [Janelia Fluor® 549]

  • beta-type platelet-derived growth factor receptor
  • CD140 antigen-like family member B
  • CD140b antigen
  • CD140b
  • EC 2.7.10
  • EC 2.7.10.1
  • JTK12
  • PDGF R beta
  • PDGFR
  • PDGFR1
  • PDGFRB
  • PDGF-R-beta
  • platelet-derived growth factor receptor, beta polypeptide

Background

Human platelet-derived growth factor (PDGF) receptor, is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-alpha and PDGF receptor-beta. PDGF receptor-beta can only bind two specific PDGF isoforms, PDGF-beta and PDGF-delta (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PDGFRB