Reactivity | Mu, Hu, RtSpecies Glossary |
Applications | WB, ELISA, Flow, IHC-Fr (-) |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Unconjugated |
Concentration | 0.5 mg/ml |
Immunogen | Peptide with sequence C-REEKLRNQRRQASN corresponding to internal region according to NP_001595.2. |
Epitope | REEKLRNQRRQASN |
Marker | Neural Marker |
Specificity | This antibody is expected to recognise both reported isoforms (NP_000271.1 and NP_001595.2). |
Predicted Species | Human (100%), Rat (100%). Backed by our 100% Guarantee. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | PAX6 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
|
Application Notes | Western blot: Approx 26kDa band observed in lysates of cell lines U251 and RAW264.7 and in nuclear lysates of cell lines K562 and HeLa, all successfully blocked by incubation with the immunising peptide (calculated MW of 24.8kDa according to Human NP_001355859.1 and 31.8 according to Mouse NP_001297075.1). Recommended concentration: 0.03-1ug/ml. Primary incubation 1 hour at room temperature. |
|
Publications |
|
Storage | Store at -20C. Avoid freeze-thaw cycles. |
Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
Preservative | 0.02% Sodium Azide |
Concentration | 0.5 mg/ml |
Purity | Immunogen affinity purified |
Publication using NB100-61654 | Applications | Species |
---|---|---|
von Holst A, Egbers U, Prochiantz A, Faissner A. Neural stem/progenitor cells express 20 tenascin C isoforms that are differentially regulated by pax6. J Biol Chem 2007-02-12 [PMID: 17264084] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Pax6 Antibody (NB100-61654)Find related products by research area.
|
Deriving neural precursor cells from human induced pluripotent stem cells By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Gene Symbol | PAX6 |
Entrez | |
Uniprot |
|