Reactivity | HuSpecies Glossary |
Applications | Flow, IHC, CyTOF-ready, KO |
Clone | PAX6/1166 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | Azide and BSA Free |
Concentration | 1.0 mg/ml |
Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44576). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
Immunogen | Recombinant fragment (N-terminus; aa 1-300) of human Pax6 protein (Uniprot: P26367) |
Localization | Nuclear |
Marker | Stem Cell Marker |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | PAX6 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
Buffer | 10 mM PBS |
Preservative | No Preservative |
Concentration | 1.0 mg/ml |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Pax6 Antibody (NBP2-47915)Find related products by research area.
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Deriving neural precursor cells from human induced pluripotent stem cells By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions... Read full blog post. |
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Gene Symbol | PAX6 |