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PAK1 Antibody

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Western Blot: PAK1 Antibody [NBP2-19680] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK1 antibody diluted at 1:1000.
Western Blot: PAK1 Antibody [NBP2-19680] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK1 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PAK1 Antibody [NBP2-19680] - Immunofluorescence analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PAK1 Antibody [NBP2-19680] - Paraffin-embedded mouse fore brain. PAK1 antibody [N2C2], Internal dilution: 1:500.
Western Blot: PAK1 Antibody [NBP2-19680] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:5000.
Western Blot: PAK1 Antibody [NBP2-19680] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.
Western Blot: PAK1 Antibody [NBP2-19680] - Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract 7.5 % SDS-PAGE PAK1 antibody [N2C2], Internal dilution: 1:1000
Immunohistochemistry-Paraffin: PAK1 Antibody [NBP2-19680] - HBL435 xenograft.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PAK1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PAK1. The exact sequence is proprietary.
Localization
Cytoplasm; Cell junction; focal adhesion
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAK1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (84%), Xenopus Laevis (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for PAK1 Antibody

  • alpha-PAK
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC130000
  • MGC130001
  • p21 protein (Cdc42/Rac)-activated kinase 1
  • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
  • p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
  • p21-activated kinase 1
  • p65-PAK
  • PAK1
  • PAK-1
  • PAKalpha
  • serine/threonine-protein kinase PAK 1
  • STE20 homolog, yeast

Background

PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAK1
Uniprot