p14ARF/CDKN2A Antibody [Biotin] Summary
Immunogen |
The epitope recognized by this antibody maps to a region between residue 82 and C-terminus (aa132) of human p14ARF using the numbering given in entry Uniprot ID Q8N726. |
Predicted Species |
Primate (100%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
CDKN2A |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA
- Immunohistochemistry-Paraffin
|
Application Notes |
This p14ARF antibody is useful for ELISA, Immunohistochemistry on paraffin-embedded sections and Western blot. In WB a band is seen at ~14 kDa representing p14ARF. *The investigator should determine the optimal working dilution for a specific application.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Readout System |
|
Reactivity Notes
Human. Based on 100% sequence identity, this antibody is predicted to react with Orangutan and White-tufted-ear marmoset.
Packaging, Storage & Formulations
Storage |
Store at 4C in the dark. |
Buffer |
PBS |
Preservative |
0.05% Sodium Azide |
Purity |
Immunogen affinity purified |
Notes
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for p14ARF/CDKN2A Antibody [Biotin]
Background
The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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FAQs for p14ARF/CDKN2A Antibody (NB200-159B) (0)
Secondary Antibodies
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