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P-Cadherin Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: P-Cadherin Antibody [NBP1-85707] - Analysis in human skin and skeletal muscle tissues. Corresponding CDH3 RNA-seq data are presented for the same tissues.
Western Blot: P-Cadherin Antibody [NBP1-85707] - Analysis in human cell line RT-4.
Immunohistochemistry-Paraffin: P-Cadherin Antibody [NBP1-85707] - Staining of human skin shows moderate membranous positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: P-Cadherin Antibody [NBP1-85707] - Staining of human colon shows weak membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: P-Cadherin Antibody [NBP1-85707] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.
Immunohistochemistry-Paraffin: P-Cadherin Antibody [NBP1-85707] - Staining of human placenta shows moderate membranous positivity in trohoblastic cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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P-Cadherin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YNGVVAYSIHSQEPKDPHDLMFTIHRSTGTISVISSGLDREKVPEYTLTIQATDMDGDGSTTTAVAVVEILDANDNAPMFDPQKYEAHVPENAVGHEVQRLTVTDLDAPNSPAWRATYLIMGGDDGDHFTITTHPE
Marker
Intercellular Junctions Marker
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDH3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
P-Cadherin Protein (NBP1-85707PEP)
Publications
Read Publications using
NBP1-85707 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for P-Cadherin Antibody

  • CAD3
  • cadherin 3, P-cadherin (placental)
  • cadherin 3, type 1, P-cadherin (placental)
  • Cadherin-3
  • CDH3
  • CDHP
  • CDHPcalcium-dependent adhesion protein, placental
  • HJMD
  • PCAD
  • PCadherin
  • P-Cadherin
  • PCADP-cadherin
  • Placental cadherin

Background

The cadherins are a family of Ca++-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. R-cadherin (for retinal-cadherin, also designated cadherin-4), which was first identified in the retina of chicken, has been shown to be involved in the development of striated muscle and potentially epithelia in addition to its involvement in retinal development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for P-Cadherin Antibody (NBP1-85707)(3)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC/IF, IF/IHC, IHC-P.


Filter By Application
ICC/IF
(1)
IF/IHC
(1)
IHC-P
(2)
All Applications
Filter By Species
Human
(2)
Rat
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using NBP1-85707 Applications Species
Bassam AM, Eesa AN et al. Expression of E-Cadherin and P-Cadherin in Breast Carcinoma. Middle-East Journal of Scientific Research. 2015-01-01 (IHC-P, Human) IHC-P Human
Yang CC, Yip HK, Chen KH et al. Impact of impaired cardiac function on the progression of chronic kidney disease---role of pharmacomodulation of valsartan.. Am J Transl Res 2017-05-31 [PMID: 28560004] (IF/IHC, ICC/IF, Rat) IF/IHC, ICC/IF Rat
Fawzy A, Allah S, Salem M, Al Farouk L Immunohistochemical and Histopathological Study of Anaplastic Lymphoma Kinase and Tyrosine-kinase Receptor Expression in Bronchogenic Carcinoma Open Access Macedonian Journal of Medical Sciences 2021-02-18 (IHC-P, Human) IHC-P Human

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDH3