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Olig2 Antibody (HL1072)

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Immunocytochemistry/ Immunofluorescence: Olig2 Antibody (HL1072) [NBP3-13712] - Olig2 antibody detects Olig2 protein at oligodendrocyte by immunofluorescent analysis. Sample: DIV10 rat cortical neuron-no AraC cells were ...read more
Immunohistochemistry-Paraffin: Olig2 Antibody (HL1072) [NBP3-13712] - Olig2 antibody [HL1072] detects Olig2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Olig2 stained by ...read more
Immunohistochemistry-Frozen: Olig2 Antibody (HL1072) [NBP3-13712] - Olig2 antibody [HL1072] detects Olig2 protein at nucleus by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: Olig2 stained by ...read more
Immunohistochemistry-Paraffin: Olig2 Antibody (HL1072) [NBP3-13712] - Olig2 antibody [HL1072] detects Olig2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Olig2 stained by ...read more
Immunohistochemistry-Paraffin: Olig2 Antibody (HL1072) [NBP3-13712] - Olig2 antibody [HL1072] detects Olig2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E10.5 embryo. Green: Olig2 ...read more
Immunohistochemistry-Paraffin: Olig2 Antibody (HL1072) [NBP3-13712] - Olig2 antibody [HL1072] detects Olig2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Olig2 stained by Olig2 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clone
HL1072
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Olig2 Antibody (HL1072) Summary

Description
Centrifuge briefly prior to opening.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Olig2. The exact sequence is proprietary.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
OLIG2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
IHC-Fr Assay dependent
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for Olig2 Antibody (HL1072)

  • BHLHB1
  • BHLHE19
  • Class B basic helix-loop-helix protein 1
  • Class E basic helix-loop-helix protein 19
  • human protein kinase C-binding protein RACK17
  • Olig2
  • OLIGO2
  • oligodendrocyte lineage transcription factor 2
  • oligodendrocyte transcription factor 2
  • oligodendrocyte-specific bHLH transcription factor 2
  • PRKCBP2
  • protein kinase C binding protein 2
  • Protein kinase C-binding protein 2
  • Protein kinase C-binding protein RACK17
  • RACK17
  • RACK17helix-loop-helix protein, class B, 1

Background

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. OIlig2 is expressed in the ventral spinal cord as early as 9.5 dpc and is scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol OLIG2