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Olig1 Antibody (257219) [Unconjugated]

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Olig1 was detected in immersion fixed paraffin-embedded sections of human glioma using 8 µg/mL Human Olig1 Monoclonal Antibody (Catalog # MAB2417) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Olig1 was detected in immersion fixed paraffin-embedded sections of human glioma using 8 µg/mL Human Olig1 Monoclonal Antibody (Catalog # MAB2417) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell ...read more
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Olig1 Monoclonal Antibody (Catalog # MAB2417) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
257219
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2417-SP
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Olig1 Antibody (257219) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Olig1
Specificity
Detects human Olig1. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Olig2 or rhOlig3.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
OLIG1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB2417 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Olig1 Antibody (257219) [Unconjugated]

  • BHLHB6
  • bHLHe21
  • Class B basic helix-loop-helix protein 6
  • Class E basic helix-loop-helix protein 21
  • helix-loop-helix protein, class B, 6
  • Olig1
  • oligo1
  • oligodendrocyte lineage transcription factor 1
  • oligodendrocyte transcription factor 1
  • oligodendrocyte-specific bHLH transcription factor 1

Background

Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in neural tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Olig1 Antibody (257219) [Unconjugated]
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Publications for Olig1 Antibody (MAB2417)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
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(1)
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(1)
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(3)
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Showing Publications 1 - 6 of 6.
Publications using MAB2417 Applications Species
A Ballester, A Guijarro, B Bravo, J Hernández, R Murillas, MI Gallego, S Ballester Hedgehog Signalling Modulates Immune Response and Protects against Experimental Autoimmune Encephalomyelitis International Journal of Molecular Sciences, 2022-03-15;23(6):. 2022-03-15 [PMID: 35328591] (IHC, Mouse) IHC Mouse
Katrin Mangold, Jan Mašek, Jingyan He, Urban Lendahl, Elaine Fuchs, Emma R. Andersson Highly efficient manipulation of nervous system gene expression with NEPTUNE Cell Reports Methods 2021-09-24 [PMID: 34557863]
S Yang, S Li, XJ Li Shortening the Half-Life of Cas9 Maintains Its Gene Editing Ability and Reduces Neuronal Toxicity Cell Rep, 2018-12-04;25(10):2653-2659.e3. 2018-12-04 [PMID: 30517854] (Western Blot, Mouse) Western Blot Mouse
Frances K. Turrell, Emma M. Kerr, Meiling Gao, Hannah Thorpe, Gary J. Doherty, Jake Cridge et al. Lung tumors with distinct p53 mutations respond similarly to p53 targeted therapy but exhibit genotype-specific statin sensitivity Genes & Development 2017-07-01 [PMID: 28790158]
Moyon S, Dubessy A, Aigrot M, Trotter M, Huang J, Dauphinot L, Potier M, Kerninon C, Melik Parsadaniantz S, Franklin R, Lubetzki C Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration. J Neurosci, 2015-01-07;35(1):4-20. 2015-01-07 [PMID: 25568099] (IHC-Fr, Mouse) IHC-Fr Mouse
Haase D, Keiner S, Mawrin C, Wolf G Reduced Morg1 expression in ischemic human brain. Neurosci. Lett., 2009-03-18;455(1):46-50. 2009-03-18 [PMID: 19429104] (IHC-P, Human) IHC-P Human

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Bioinformatics

Gene Symbol OLIG1