Home » Oct-3/4 » Oct-3/4 Antibodies » Oct-3/4 Antibody (240408) [Phycoerythrin]
Novus Biologicals products are now on bio-techne.com

Oct-3/4 Antibody (240408) [Phycoerythrin]

 (14 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To ...read more
Mouse embryonic stem cells treated with 5 µM Retinoic Acid for 3 days were stained with Rat Anti-Human/Mouse Oct-3/4 PE-conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
240408
Clonality
Monoclonal
Host
Rat
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC1759U-100UG
Alexa Fluor 405
IC1759V-100UG
Alexa Fluor 488
IC1759G-100UG
Alexa Fluor 532
FAB1759X
Alexa Fluor 594
IC1759T-100UG
Alexa Fluor 647
IC1759R-100UG
Alexa Fluor 700
IC1759N-100UG
Alexa Fluor 750
IC1759S-100UG
Allophycocyanin
IC1759A-025
Allophycocyanin
IC1759A-100
Biotin
FAB1759B
CoraFluor 1
FAB1759CL1
DyLight 350
FAB1759D
DyLight 405
FAB1759E
DyLight 488
FAB1759K
DyLight 550
FAB1759L
DyLight 594
FAB1759M
DyLight 650
FAB1759W
DyLight 680
FAB1759Y
DyLight 755
FAB1759Z
Fluorescein
IC1759F-100
Fluorescein
IC1759F-025
HRP
FAB1759H
Janelia Fluor 525
FAB1759JF525
Janelia Fluor 549
FAB1759I
Janelia Fluor 585
FAB1759JF585
Janelia Fluor 635
FAB1759JF635
Janelia Fluor 646
FAB1759J
Janelia Fluor 669
FAB1759JF669
Phycoerythrin
IC1759P-025
Unconjugated
MAB1759
mFluor Violet 450 SE
FAB1759MFV450
mFluor Violet 500 SE
FAB1759MFV500
mFluor Violet 610 SE
FAB1759MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1759
Carrier Free
MAB1759
Carrier Free
MAB1759-SP
Carrier Free
IC1759A-100
Carrier Free
IC1759A-025
Carrier Free
IC1759F-100
Carrier Free
IC1759F-025
Carrier Free
IC1759P-025
Carrier Free
IC1759V-100UG
Carrier Free
IC1759U-100UG
Carrier Free
IC1759T-100UG
Carrier Free
IC1759S-100UG
Carrier Free
IC1759R-100UG
Carrier Free
IC1759N-100UG
Carrier Free
IC1759G-100UG
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Oct-3/4 Antibody (240408) [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Specificity
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
POU5F1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC1759P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oct-3/4 Antibody (240408) [Phycoerythrin]

  • 3-Oct
  • Oct-3/4
  • OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1
  • Oct4
  • Otf3g
  • Otf4
  • Pou5f1

Background

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells (1- 3). Oct-3/4 is required to sustain stem cell self-renewal and pluripotency (4). It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

  1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Nanog Antibody
[Unconjugated]
AF1997
Chromatin Immunoprecipitation (ChIP) Nanog Antibody [Unconjugated]
Immunocytochemistry Nanog Antibody [Unconjugated]
Immunocytochemistry Nanog Antibody [Unconjugated]
(5 Reviews)
(301 Publications)
Species: Hu
Applications: ChIP, ICC, IHC, WB
NANOGP8 Antibody
H00388112-B01P
Western Blot NANOGP8 Antibody
Species: Hu
Applications: WB
SOX2 Antibody
[Unconjugated]
AF2018
Western Blot SOX2 Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) SOX2 Antibody [Unconjugated]
Simple Western SOX2 Antibody [Unconjugated]
(6 Reviews)
(354 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, ICC, IHC, Simple Western, WB
c-Myc Antibody
(9E10) - BSA Free
NB600-302
Flow Cytometry c-Myc Antibody (9E10) - BSA Free
Flow Cytometry c-Myc Antibody (9E10) - BSA Free
Western Blot c-Myc Antibody (9E10) - BSA Free
(4 Reviews)
(46 Publications)
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
KLF4 Antibody
[Unconjugated]
AF3158
Chromatin Immunoprecipitation (ChIP) KLF4 Antibody [Unconjugated]
Immunocytochemistry KLF4 Antibody [Unconjugated]
Immunohistochemistry KLF4 Antibody [Unconjugated]
(2 Reviews)
(86 Publications)
Species: Mu
Applications: ChIP, ICC, IHC, WB
LIF [Unconjugated]
7734-LF
LIF [Unconjugated]
(30 Publications)
Species: Hu
Applications: BA
Rex-1/ZFP42
Antibody (5E11A6) - B ...
NBP2-37357
ELISA Rex-1/ZFP42 Antibody (5E11A6) - BSA Free
Western Blot Rex-1/ZFP42 Antibody (5E11A6) - BSA Free
Western Blot Rex-1/ZFP42 Antibody (5E11A6) - BSA Free
(1 Publication)
Species: Hu, Mu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
Nestin Antibody
(196908) [Unconjugate
MAB1259
Immunocytochemistry Nestin Antibody (196908) [Unconjugated]
Intracellular Staining by Flow Cytometry Nestin Antibody (196908) [Unconjugated]
Western Blot Nestin Antibody (196908) [Unconjugated]
(3 Reviews)
(158 Publications)
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
CD117/c-kit
Antibody [Unconjugate
AF1356
Simple Western CD117/c-kit Antibody [Unconjugated]
Western Blot CD117/c-kit Antibody [Unconjugated]
Immunohistochemistry CD117/c-kit Antibody [Unconjugated]
(4 Reviews)
(110 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, Simple Western, WB
FGF-4
[Unconjugated]
7460-F4/CF
FGF-4 [Unconjugated]
(2 Publications)
Species: Hu
Applications: BA
Steroid sulfatase
Antibody
NBP1-90095
Immunohistochemistry-Paraffin Steroid sulfatase Antibody
Immunohistochemistry-Paraffin Steroid sulfatase Antibody
Immunohistochemistry-Paraffin Steroid sulfatase Antibody
(3 Publications)
Species: Hu
Applications: IHC, IHC-P
Oct-3/4 Antibody
[Unconjugated]
AF1759
Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]
Immunocytochemistry Oct-3/4 Antibody [Unconjugated]
Simple Western Oct-3/4 Antibody [Unconjugated]
(73 Publications)
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
LIN-28A Antibody
[Unconjugated]
AF3757
Immunocytochemistry LIN-28A Antibody [Unconjugated]
Immunocytochemistry LIN-28A Antibody [Unconjugated]
Western Blot LIN-28A Antibody [Unconjugated]
(1 Review)
(22 Publications)
Species: Hu
Applications: ICC, WB
CD133 Antibody -
BSA Free
NB120-16518
Immunohistochemistry-Paraffin CD133 Antibody - BSA Free
Western Blot CD133 Antibody - BSA Free
Chromatin Immunoprecipitation CD133 Antibody - BSA Free
(40 Publications)
Species: Hu, Mu, Po, Rt
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
CD44 Antibody
(2C5) [Unconjugated] ...
BBA10
Simple Western CD44 Antibody (2C5) [Unconjugated] - s Pan Specific
Western Blot CD44 Antibody (2C5) [Unconjugated] - s Pan Specific
Immunohistochemistry CD44 Antibody (2C5) [Unconjugated] - s Pan Specific
(5 Reviews)
(49 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(532 Publications)
Species: Hu
Applications: BA
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB

Publications for Oct-3/4 Antibody (IC1759P)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.


Filter By Application
Flow Cytometry
(10)
ICC
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(8)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using IC1759P Applications Species
F Vlahova, KE Hawkins, AM Ranzoni, KL Hau, R Sagar, P Coppi, AL David, J Adjaye, PV Guillot Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A Sci Rep, 2019-05-31;9(1):8126. 2019-05-31 [PMID: 31148575] (Flow Cytometry, ICC, Western Blot, Human) Flow Cytometry, ICC, Western Blot Human
M Dejosez, TP Zwaka Generation of hiPSTZ16 (ISMMSi003-A) cell line from normal human foreskin fibroblasts Stem Cell Res, 2017-12-02;26(0):44-46. 2017-12-02 [PMID: 29227831] (Flow Cytometry, Human) Flow Cytometry Human
Vincent Le Coz, Chaobin Zhu, Aurore Devocelle, Aimé Vazquez, Claude Boucheix, Sandy Azzi et al. IGF-1 contributes to the expansion of melanoma-initiating cells through an epithelial-mesenchymal transition process Oncotarget 12/13/2016 [PMID: 27764776]
A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells PLoS ONE, 2016-09-08;11(9):e0161229. 2016-09-08 [PMID: 27606941] (Flow Cytometry, Human) Flow Cytometry Human
Behnam Ahmadian Baghbaderani, Adhikarla Syama, Renuka Sivapatham, Ying Pei, Odity Mukherjee, Thomas Fellner et al. Detailed Characterization of Human Induced Pluripotent Stem Cells Manufactured for Therapeutic Applications Stem Cell Reviews and Reports 8/1/2016 [PMID: 27283945]
Hasmim Meriem, Bruno Stefania, Azzi Sandy et al. Isolation and characterization of renal cancer stem cells from patient-derived xenografts. Oncotarget 1/1/2016 [PMID: 26551931]
Sztiller-Sikorska M, Hartman M, Talar B, Jakubowska J, Zalesna I, Czyz M Phenotypic diversity of patient-derived melanoma populations in stem cell medium. Lab Invest, 2015-04-13;95(6):672-83. 2015-04-13 [PMID: 25867763] (Flow Cytometry, Human) Flow Cytometry Human
Nie Y, Walsh P, Clarke D, Rowley J, Fellner T Scalable passaging of adherent human pluripotent stem cells. PLoS ONE, 2014-01-30;9(1):e88012. 2014-01-30 [PMID: 24498239] (Flow Cytometry, Human) Flow Cytometry Human
Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, Song J, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak E, Jonkers J, Berns A Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells. EMBO Mol Med, 2014-01-08;6(2):212-25. 2014-01-08 [PMID: 24401838] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Tea Soon Park, Ludovic Zimmerlin, Elias T. Zambidis Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells Cytometry Part A 1/1/2013 [PMID: 22736485]
Show All 14 Publications.

Reviews for Oct-3/4 Antibody (IC1759P) (0)

There are no reviews for Oct-3/4 Antibody (IC1759P). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Oct-3/4 Antibody (IC1759P) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Oct-3/4 Products

Array IC1759P

Blogs on Oct-3/4

There are no specific blogs for Oct-3/4, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows lysates of D3 mouse embryonic stem cell line, NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SOX2 Antibody [Unconjugated]
AF2018

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Oct-3/4 Antibody (240408) [Phycoerythrin] and receive a gift card or discount.


Bioinformatics

Gene Symbol POU5F1
Uniprot