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Oct-3/4 Antibody (240408) [Fluorescein]

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BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1759F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1759F, filled histogram) or isotype control antibody (Catalog # IC013F, open ...read more
Mouse embryonic stem cells treated with 5 µM Retinoic Acid for 2 days were stained with Rat Anti-Human/Mouse Oct-3/4 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1759F, filled histogram) or isotype control ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
240408
Clonality
Monoclonal
Host
Rat
Conjugate
Fluorescein

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC1759U-100UG
Alexa Fluor 405
IC1759V-100UG
Alexa Fluor 488
IC1759G-100UG
Alexa Fluor 532
FAB1759X
Alexa Fluor 594
IC1759T-100UG
Alexa Fluor 647
IC1759R-100UG
Alexa Fluor 700
IC1759N-100UG
Alexa Fluor 750
IC1759S-100UG
Allophycocyanin
IC1759A-025
Allophycocyanin
IC1759A-100
Biotin
FAB1759B
CoraFluor 1
FAB1759CL1
DyLight 350
FAB1759D
DyLight 405
FAB1759E
DyLight 488
FAB1759K
DyLight 550
FAB1759L
DyLight 594
FAB1759M
DyLight 650
FAB1759W
DyLight 680
FAB1759Y
DyLight 755
FAB1759Z
Fluorescein
IC1759F-025
HRP
FAB1759H
Janelia Fluor 525
FAB1759JF525
Janelia Fluor 549
FAB1759I
Janelia Fluor 585
FAB1759JF585
Janelia Fluor 635
FAB1759JF635
Janelia Fluor 646
FAB1759J
Janelia Fluor 669
FAB1759JF669
Phycoerythrin
IC1759P-025
Phycoerythrin
IC1759P-100
Unconjugated
MAB1759
mFluor Violet 450 SE
FAB1759MFV450
mFluor Violet 500 SE
FAB1759MFV500
mFluor Violet 610 SE
FAB1759MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1759
Carrier Free
MAB1759
Carrier Free
MAB1759-SP
Carrier Free
IC1759A-100
Carrier Free
IC1759A-025
Carrier Free
IC1759F-025
Carrier Free
IC1759P-100
Carrier Free
IC1759P-025
Carrier Free
IC1759V-100UG
Carrier Free
IC1759U-100UG
Carrier Free
IC1759T-100UG
Carrier Free
IC1759S-100UG
Carrier Free
IC1759R-100UG
Carrier Free
IC1759N-100UG
Carrier Free
IC1759G-100UG
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Oct-3/4 Antibody (240408) [Fluorescein] Summary

Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Specificity
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
POU5F1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC1759F in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oct-3/4 Antibody (240408) [Fluorescein]

  • 3-Oct
  • Oct-3/4
  • OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1
  • Oct4
  • Otf3g
  • Otf4
  • Pou5f1

Background

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

  1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Oct-3/4 Antibody (IC1759F)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(3)
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Human
(3)
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Showing Publications 1 - 5 of 5.
Publications using IC1759F Applications Species
R Núñez-Told, E Martínez-S, C Gil-Recio, MÁ Carrasco, A Al Madhoun, S Montori, M Atari Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation BMC Cell Biol., 2017-04-21;18(1):21. 2017-04-21 [PMID: 28427322] (Flow Cytometry, Human) Flow Cytometry Human
Wai-Kin Yu Chemoresistant lung cancer stem cells display high DNA repair capability to remove cisplatin-DNA damage Br. J. Pharmacol, 2017-01-16;0(0):. 2017-01-16 [PMID: 27933604] (Flow Cytometry, Human) Flow Cytometry Human
K Tilgner, I Neganova, I Moreno-Gimeno, J Y AL-Aama, D Burks, S Yung et al. A human iPSC model of Ligase IV deficiency reveals an important role for NHEJ-mediated-DSB repair in the survival and genomic stability of induced pluripotent stem cells and emerging haematopoietic progenitors Cell Death & Differentiation 2013-08-01 [PMID: 23722522]
Jörg C. Gerlach, Marc Lübberstedt, Josefina Edsbagge, Alexander Ring, Mariah Hout, Matt Baun et al. Interwoven Four-Compartment Capillary Membrane Technology for Three-Dimensional Perfusion with Decentralized Mass Exchange to Scale Up Embryonic Stem Cell Culture Cells Tissues Organs 2010-01-01 [PMID: 20197653]
Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C Automated, scalable culture of human embryonic stem cells in feeder-free conditions. Biotechnol. Bioeng., 2009-04-15;102(6):1636-44. 2009-04-15 [PMID: 19062183] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol POU5F1
Uniprot