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OBSCN Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818] - Analysis in human skeletal muscle and liver tissues. Corresponding OBSCN RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: OBSCN Antibody [NBP1-88818] - Staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

OBSCN Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ACVVVSGSESEAESSSGGELDDAFRRAARRLHRLFRTKSPAEVSDEELFLSADEGPAEPEEPADWQTYREDEHFICIRFEALTEARQAVTRFQEMFATLGIGVEIKLVEQGPRRVEMCISKETPAPVVPPEPLPSLLTSDAAP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OBSCN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
OBSCN Protein (NBP1-88818PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for OBSCN Antibody

  • ARHGEF30
  • DKFZp666E245
  • EC 2.7.11.1
  • FLJ14124
  • KIAA1556MGC120412
  • KIAA1639MGC138590
  • MGC120409
  • MGC120410
  • MGC120411
  • obscurin
  • obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
  • obscurin, myosin light chain kinase
  • obscurin-MLCK
  • Obscurin-myosin light chain kinase
  • obscurin-RhoGEF
  • UNC89

Background

OBSCN - obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OBSCN Antibody (NBP1-88818) (0)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for OBSCN Antibody (NBP1-88818) (0)

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Secondary Antibodies

 

Isotype Controls

Additional OBSCN Products

Array NBP1-88818

Research Areas for OBSCN Antibody (NBP1-88818)

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Blogs on OBSCN

There are no specific blogs for OBSCN, but you can read our latest blog posts.
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Bioinformatics

Gene Symbol OBSCN