![Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Western-Blot-NBP2-33863-img0003.jpg "Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: ERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTNLVECLTKINRMDIVHLMETNTEPLQERISHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEQAVSKESETC |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ANK2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-P Retrieval method: HIER pH6. ICC/IF Fixation/Permeabilization: PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Ankyrin Brain Antibody (NBP2-33863)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cell line RH-30 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33863-img0008.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cell line RH-30 shows localization to plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0012.jpg "Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human placenta shows no positivity in trophoblastic cells as expected.")
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0004.jpg "Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows high expression.")
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0005.jpg "Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human pancreas shows low expression as expected.")
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Analysis in human cerebral cortex and skin tissues using NBP2-33863 antibody. Corresponding ANK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0009.jpg "Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Analysis in human cerebral cortex and skin tissues using NBP2-33863 antibody. Corresponding ANK2 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0010.jpg "Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows strong positivity in neuropil.")
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0011.jpg "Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0013.jpg "Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human skin shows no positivity in squamous epithelial cells as expected.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")