![Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Western-Blot-NBP3-05549-img0002.jpg "Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.")
| Reactivity | Hu, Mu, Rt, Bv, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Chicken |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | The C-terminal 398 amino acids of human Ankyrin 3 expressed in and purified from E. coli. |
| Isotype | IgY |
| Clonality | Polyclonal |
| Host | Chicken |
| Gene | ANK3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol. Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY. |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Ankyrin 3 Antibody (NBP3-05549)Find related products by research area.
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| Gene Symbol | ANK3 |
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05549] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Ankyrin 3 Antibody, CPCA-ANK3, at a dilution of 1:2,000 in green, and costained with mouse mAb to RBFOX3/NeuN at a dilution of 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The Ankyrin 3 antibody stains the axonal initial segments, while the RBFOX3/NeuN antibody reveals perikarya and nuclei of neurons.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05549-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05549] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Ankyrin 3 Antibody, CPCA-ANK3, at a dilution of 1:2,000 in green, and costained with mouse mAb to RBFOX3/NeuN at a dilution of 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The Ankyrin 3 antibody stains the axonal initial segments, while the RBFOX3/NeuN antibody reveals perikarya and nuclei of neurons.")
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