![Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Western blot analysis of Myosin light chain kinase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Western-Blot-NBP2-67486-img0010.jpg "Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Western blot analysis of Myosin light chain kinase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | SU40-06 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Myosin light chain kinase aa 1,865-1,914 / 1,914. (SwissProt: Q15746 Human; SwissProt: Q6PDN3 Mouse; SwissProt: D3ZFU9 Rat) |
| Localization | Cytoplasm, Cell projection, Cleavage furrow, Cytoskeleton. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MYLK |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Myosin light chain kinase Antibody (NBP2-67486)Find related products by research area.
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| Gene Symbol | MYLK |
![Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunocytochemistry-Immunofluorescence-NBP2-67486-img0009.jpg "Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded rat smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunohistochemistry-Paraffin-NBP2-67486-img0007.jpg "Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded rat smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.")
![Flow Cytometry: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of SH-SY-5Y cells with MYLK antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Flow-Cytometry-NBP2-67486-img0001.jpg "Flow Cytometry: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of SH-SY-5Y cells with MYLK antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of MYLK on human lung lysates using anti-MYLK antibody at 1/5,000 dilution.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Western-Blot-NBP2-67486-img0008.jpg "Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of MYLK on human lung lysates using anti-MYLK antibody at 1/5,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunocytochemistry-Immunofluorescence-NBP2-67486-img0002.jpg "Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunocytochemistry-Immunofluorescence-NBP2-67486-img0003.jpg "Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunocytochemistry-Immunofluorescence-NBP2-67486-img0004.jpg "Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded human colon cancer tissue using anti-MYLK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunohistochemistry-Paraffin-NBP2-67486-img0005.jpg "Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded human colon cancer tissue using anti-MYLK antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-light-chain-kinase-Antibody-SU40-06-Immunohistochemistry-Paraffin-NBP2-67486-img0006.jpg "Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.")
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![Western Blot MRCL3 [p Ser19, p Ser20] Antibody](https://images.novusbio.com/images/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0019.jpg)
![Immunohistochemistry MRCL3 [p Ser19, p Ser20] Antibody](https://images.novusbio.com/images/nb100-1687_rabbit-polyclonal-mrcl3-p-ser19-p-ser20-antibody-23520231814583.jpg)
![Immunohistochemistry MRCL3 [p Ser19, p Ser20] Antibody](https://images.novusbio.com/images/nb100-1687_rabbit-polyclonal-mrcl3-p-ser19-p-ser20-antibody-235202318143193.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")