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NuMA Antibody (SR1359)

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Western Blot-NBP3-22672-NuMA Antibody (SR1359)-Analysis of Mad2L1 expression in HeLa cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
SR1359
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NuMA Antibody (SR1359) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human NuMA (Uniprot #: Q14980)
Specificity
Detects endogenous levels of total NuMA
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NUMA1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
238 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for NuMA Antibody (SR1359)

  • centrophilin stabilizes mitotic spindle in mitotic cells
  • NMP-22
  • nuclear mitotic apparatus protein 1
  • NuMA protein
  • NuMA
  • NUMA1
  • SP-H Antigen
  • structural nuclear protein

Background

NuMA (nuclear mitotic apparatus) is a long coiled-coil protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning. Therefore, this antibody is useful as a cell cycle marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NuMA Antibody (NBP3-22672) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NuMA Antibody (NBP3-22672) (0)

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Secondary Antibodies

 

Isotype Controls

Additional NuMA Products

Research Areas for NuMA Antibody (NBP3-22672)

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Blogs on NuMA.

NuMA: The Key to Asymmetric Cell Division
Nuclear Mitotic Apparatus protein (NuMA) is a cell cycle-related protein that acts as an organizer of the mitotic spindle during mitosis. It may be involved in coordinating the alignment of the mitotic spindle to the cellular polarity axis, which is a...  Read full blog post.

Nucleus and Mitotic Apparatus (NuMA) Protein in Cell Cycle Regulation
NuMA, the major protein of the "Nucleus and Mitotic Apparatus", is a structural protein in vertebrates involved in cell cycle regulation. It localizes to the nucleus during interphase, and accumulates at the spindle poles during mitosis and NuMA has b...  Read full blog post.

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Bioinformatics

Gene Symbol NUMA1