In what assays cross-reactivity of NB500-179 with mouse has been confirmed? Do you have any information, or are there by chance any mouse images that just haven't made it onto the datasheet yet?

Name: TPX2 Antibody
Brand: Novus Biologicals
SKU: NB500-179
Rating: 5 (1 reviews)

Question

Accepted Answer

We tested this ab on mouse cells in a Western blot with good results.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Po, MaSpecies Glossary
Applications	WB, Simple Western, Flow, ICC/IF, IP, KD
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated

Immunogen	A recombinant segment of the C-terminal domain of human TPX2 [UniProt# Q9ULW0]
Localization	Nuclear. Staining of non-mitotic cells and cell cycle-dependent changes in abundance peaking at G2/M phase.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	TPX2
Purity	Unpurified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	Whole antisera
Preservative	No Preservative
Purity	Unpurified

Publications using NB500-179	Applications	Species
Asteriti IA, Polverino F, Stagni V et al. AurkA nuclear localization is promoted by TPX2 and counteracted by protein degradation Life science alliance 2023-05-01 [PMID: 36797043] (WB, ICC/IF, Human) Details: Dilution used in ICC/IF 1:1,500 WB 1:1,000	WB, ICC/IF	Human
Wu J, Larreategui-Aparicio A, Lambers MLA et al. Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression Current Biology 2023-03-13 [PMID: 36720222]
Chu TLH Aurora kinase A, regulated by HMMR and TPX2, controls cell migration and engraftment via centrosome polarization Thesis 2020-01-01 (WB, ICC/IF, Human, Mouse)	WB, ICC/IF	Human, Mouse
Hitakomate E, Koontongkaew S Differential expression of Ran-binding protein 2 (RanBP2) and Targeting Protein for Xklp2 (TPX2) in head and neck squamous cell carcinoma cells Genomics and Genetics 2019-12-14 (WB, Human)	WB	Human
Cornago Protomartir M. Caracterizacion de los efectos de los inhibidores de las histona desacetilasas en lineas celulares de glioma Thesis. 2014-09-01 (WB, ICC/IF, Human)	WB, ICC/IF	Human
Tang X, Wei W, Snowball JM et al. EMC3 regulates mesenchymal cell survival via control of the mitotic spindle assembly iScience 2022-11-01 [PMID: 36624844]
Oh W, Wu TT, Jeong SY et al. CtIP Regulates Mitotic Spindle Assembly by Modulating the TPX2-Aurora A Signaling Axis Cells 2022-09-08 [PMID: 36139389] (WB, IP)	WB, IP
Li XQ, Wang Y, Yang SJ et al. Melatonin protects against maternal diabetes-associated meiotic defects by maintaining mitochondrial function Free radical biology & medicine 2022-08-01 [PMID: 35792241] (FLOW, Mouse)	FLOW	Mouse
Felix Orben, Katharina Lankes, Christian Schneeweis, Zonera Hassan, Hannah Jakubowsky, Lukas Krau beta , Fabio Boniolo, Carolin Schneider, Arlett Schäfer, Janine Murr, Christoph Schlag, Bo Kong, Rupert Öllinger, Chengdong Wang, Georg Beyer, Ujjwal M. Mahajan, Yonggan Xue, Julia Mayerle, Roland M. Schmid, Bernhard Kuster, Roland Rad, Christian J. Braun, Matthias Wirth, Maximilian Reichert, Dieter Saur, Günter Schneider Epigenetic drug screening defines a PRMT5 inhibitor–sensitive pancreatic cancer subtype JCI Insight 2022-05-23 [PMID: 35439169]
Jabali A, Hoffrichter A, Uzquiano A et al. Human cerebral organoids reveal progenitor pathology in EML1-linked cortical malformation EMBO reports 2022-03-15 [PMID: 35289477] (ICC/IF, Human)	ICC/IF	Human
Zhang YL, Zheng W, Ren P Et al. Biallelic mutations in MOS cause female infertility characterized by human early embryonic arrest and fragmentation EMBO molecular medicine 2021-11-15 [PMID: 34779126] (ICC/IF, Mouse)	ICC/IF	Mouse
Sawada JI, Matsuno K, Ogo N, Asai A Various effects of two types of kinesin-5 inhibitors on mitosis and cell proliferation Biochemical pharmacology 2021-09-25 [PMID: 34582773] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Das R, SjOstrOm M, Shrestha R Et al. An integrated functional and clinical genomics approach reveals genes driving aggressive metastatic prostate cancer Nature communications 2021-07-29 [PMID: 34326322] (WB, Mouse)	WB	Mouse
Wang X, Baumann C, De La Fuente R, Viveiros MM Loss of acentriolar MTOCs disrupts spindle pole Aurora A and assembly of the liquid-like meiotic spindle domain in oocytes Journal of cell science 2021-07-15 [PMID: 34152366]
Wang R, Abdelbaki A, Ascanelli C et al. Selective targeting of non-centrosomal AURKA functions through use of a novel targeted protein degradation tool Commun Biol 2021-05-29 [PMID: 34050235]
Zhang YY, Dong LX, Bao HL et al. Inhibition of interleukin-1 beta plays a protective role in Alzheimer's disease by promoting microRNA-9-5p and downregulating targeting protein for xenopus kinesin-like protein 2 International immunopharmacology 2021-04-20 [PMID: 33892301]
Polverino F, Naso F, Palmerini V et al. The Aurora-A/TPX2 Axis Directs Spindle Orientation by Regulating NuMa and Microtubule Dynamics Curr Biol 2020-12-04 [PMID: 33275894]
Naso FD, Sterbini V, Crecca E et al. Excess TPX2 Interferes with Microtubule Disassembly and Nuclei Reformation at Mitotic Exit Cells 2020-02-06 [PMID: 32041138] (WB, Human)	WB	Human
Drutovic D, Duan X, Li R et al. RanGTP and importin beta regulate meiosis I spindle assembly and function in mouse oocytes EMBO J. 2019-10-16 [PMID: 31617608] (ICC/IF, Mouse)	ICC/IF	Mouse
Capalbo L, Bassi ZI, Geymonat M et al. The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis Nat Commun 2019-10-04 [PMID: 31586073] (WB, KD, Human)	WB, KD	Human
So C, Seres K. B, et al. A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science 2019-06-28 [PMID: 31249032] (ICC/IF, Sheep, Mouse, Bovine, Porcine)	ICC/IF	Sheep, Mouse, Bovine, Porcine
van Gijn SE, Wierenga E, van den Tempel N et al. TPX2/Aurora kinase A signaling as a potential therapeutic target in genomically unstable cancer cells. Oncogene 2018-09-03 [PMID: 30177840] (WB, Human)	WB	Human
Mann BJ, Wadsworth P. Distribution of Eg5 and TPX2 in Mitosis: Insight from CRISPR tagged Cells Cytoskeleton (Hoboken) 2018-08-19 [PMID: 30123975] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Hsu WH, Wang WJ, Lin WY et al. Adducin-1 is essential for spindle pole integrity through its interaction with TPX2 EMBO Rep. 2018-06-19 [PMID: 29925526] (WB, Human)	WB	Human
Manukyan A, Sargsyan L, Parsons SJ, Stukenberg PT. P190RhoGAP prevents mitotic spindle fragmentation and is required to activate Aurora A kinase at acentriolar poles Chromosoma 2018-04-14 [PMID: 29656322]
Park J, Kwon MS, Kim EE, Lee H. USP35 regulates mitotic progression by modulating the stability of Aurora B. Nat Commun. 2018-02-15 [PMID: 29449677] (WB, Human)	WB	Human
Connell M, Chen H, Jiang J et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development Elife. 2017-10-10 [PMID: 28994651] (Mouse)		Mouse
Mann BJ, Balchand SK, Wadsworth P. Regulation of Kif15 localization and motility by the C-terminus of TPX2 and microtubule dynamics. Mol. Biol. Cell. 2016-11-16 [PMID: 27852894]
Lee SY, Kim EY, Kim KH, Lee KA Bcl2l10, a new Tpx2 binding partner, is a master regulator of Aurora kinase A in mouse oocytes Cell Cycle. 2016-10-17 [PMID: 27753540] (WB, Mouse)	WB	Mouse
Rao SR, Flores-Rodriguez N, Page SL et al. The clathrin-dependent spindle proteome. Mol. Cell Proteomics. 2016-05-12 [PMID: 27174698] (ICC/IF, Human)	ICC/IF	Human
Franciosi F, Manandhar S, Conti M. FSH Regulates mRNA Translation in Mouse Oocytes and Promotes Developmental Competence. Endocrinology 2016-02-01 [PMID: 26653334] (Mouse)		Mouse
Winkler C, De Munter S, Van Dessel N et al. The selective inhibition of protein phosphatase-1 results in mitotic catastrophe and impaired tumor growth. J. Cell. Sci. 2015-11-05 [PMID: 26542020] (WB, Human)	WB	Human
Barr AR, Bakal C. A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly. Sci Rep 2015-01-01 [PMID: 26037491] (WB, Human)	WB	Human
Cornago M, Garcia-Alberich C, Blasco-Angulo N et al. Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe. Cell Death Dis. 2014-10-03 [PMID: 25275596] (WB, ICC/IF, Human) Details: Survivin antibody used for ICC-IF on U251-MG cells in metaphase after HDAC inhibitor suberanilohydroxamic acid/SAHA treatment (Figure 7a). Antibody also used for WB on lysates of glioma cell lines U87, U251-MG, LN229 cells as well as human GBM primary cultures C55, C65, C48 and C60 treated or not with HDAC inhibitor SAHA and valproic acid/VPA (Figure 7b and c)	WB, ICC/IF	Human
Meyer HJ, Rape M. Enhanced protein degradation by branched ubiquitin chains. Cell. 2014-05-08 [PMID: 24813613] (ICC/IF, Human)	ICC/IF	Human
Song L, Craney A, Rape M. Microtubule-dependent regulation of mitotic protein degradation. Mol. Cell 2014-01-27 [PMID: 24462202] (WB, Human)	WB	Human
Chen J, Torcia S, Xie F et al. Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes. Nat Cell Biol. 2013-12-01 [PMID: 24270888] (WB, Mouse)	WB	Mouse
Jiang J, Mohan P, Maxwell CA. The Cytoskeletal Protein RHAMM and ERK1/2 Activity Maintain the Pluripotency of Murine Embryonic Stem Cells. PLoS One. 2013-09-03 [PMID: 24019927] (FLOW, Mouse)	FLOW	Mouse
Regan JL, Sourisseau T, Soady K et al. Aurora a kinase regulates mammary epithelial cell fate by determining mitotic spindle orientation in a notch-dependent manner. Cell Rep 2013-07-11 [PMID: 23810554] (ICC/IF, Mouse)	ICC/IF	Mouse
Park MT, Oh ET, Song MJ et al. NQO1 prevents radiation-induced aneuploidy by interacting with Aurora-A. Carcinogenesis 2013-06-26 [PMID: 23803694] (ICC/IF, WB, IP, Human)	ICC/IF, WB, IP	Human
Hasegawa K, Ryu SJ, Kalab P. Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells. J Cell Biol 2013-01-21 [PMID: 23319601] (WB, Human)	WB	Human
Roscioli E, Di Francesco L, Bolognesi A et al. Importin-beta negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores. J Cell Biol 2012-02-20 [PMID: 22331847] (Human)		Human
Ma N, Tulu US, Ferenz NP et al. Poleward transport of TPX2 in the mammalian mitotic spindle requires dynein, Eg5, and microtubule flux Mol Biol Cell 2010-03-15 [PMID: 20110350] (ICC/IF, Porcine)	ICC/IF	Porcine
Williamson A, Wickliffe KE, Mellone BG et al. Identification of a physiological E2 module for the human anaphase-promoting complex. PNAS;106(43):18213-18218. 2009-01-01 [PMID: 19822757]
Bird, AW, Hyman, AA. Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A. J Cell Biol;182(2):289-300. 2008-07-28 [PMID: 18663142]
Xia F, Canovas PM, Guadagno TM et al. A survivin-ran complex regulates spindle formation in tumor cells. Mol Cell Biol 28(17):5299-311. 2008-09-01 [PMID: 18591255] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Xia F, Lee CW, Altieri DC. Tumor cell dependence on Ran-GTP-directed mitosis. Cancer Res 68(6):1826-33. 2008-03-15 [PMID: 18339863] (ICC/IF, Human)	ICC/IF	Human
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Gene Symbol	TPX2
Entrez	22974(Human) 72119(Mouse) 311546(Rat)
Uniprot	Q9ULW0(Human)

	Patrizia Lavia 01/16/2015
Application:	IF
Species:	Human