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KIFC1 Antibody

Images

 
Immunohistochemistry-Paraffin: KIFC1 Antibody [NB100-40844] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- KIFC1/MKLP2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: KIFC1 Antibody [NB100-40844] - Detection of Human KIFC1/MKLP2 on HeLa whole cell lysate using NB100-40844. KIFC1/MKLP2 was also immunoprecipitated by other rabbit anti-KIFC1/MKLP2 antibodies and ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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KIFC1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 625 and 673 of human Kinesin Family Member C1 (Mitotic Kinesin-Like Protein 2) using the numbering given in entry NP_002254.1 (GeneID 3833).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIFC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. WB reactivity reported in (PMID: 28430595). Western Blot Not recommended. Chip, WB reactivity reported in (PMID: 24648347). Use in ICC/IF was reported in scientific literature (PMID: 30804089).
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-40844 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30804089).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KIFC1 Antibody

  • HSETMGC1202
  • kinesin family member C1
  • kinesin-like 2
  • Kinesin-like protein 2
  • kinesin-like protein KIFC1
  • Kinesin-related protein HSET
  • KNSL2MGC149736
  • MGC149737

Background

KIFC1 is a C-terminal kinesin motor associated with the nuclear membrane and acrosome in round and elongating spermatids. This location in developing spermatids is consistent with possible roles in acrosome elongation and manchette motility or both. It is a minus-end-directed motor enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KIFC1 Antibody (NB100-40844)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KIFC1 Antibody (NB100-40844) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on KIFC1

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Bioinformatics

Gene Symbol KIFC1
Entrez
Uniprot