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RCC1 Antibody

Images

 
Independent Antibodies: Western Blot: RCC1 Antibody [NBP1-85637] - Analysis using Anti-RCC1 antibody NBP1-85637 (A) shows similar pattern to independent antibody NBP1-85638 (B).
Immunocytochemistry/ Immunofluorescence: RCC1 Antibody [NBP1-85637] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RCC1 Antibody [NBP1-85637] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: RCC1 Antibody [NBP1-85637] - Staining of human testis shows strong nuclear positivity in spermatocytes and spermatids.
Immunohistochemistry-Paraffin: RCC1 Antibody [NBP1-85637] - Staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RCC1 Antibody [NBP1-85637] - Staining of human prostate shows moderate nuclear psotivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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RCC1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MVPGKVELQEKVVQVSAGDSHTAALTDDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVM
Predicted Species
Mouse (97%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RCC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RCC1 Protein (NBP1-85637PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RCC1 Antibody

  • Cell cycle regulatory protein
  • CHC1RCC1-I
  • chromosome condensation 1
  • Chromosome condensation protein 1
  • guanine nucleotide-releasing protein
  • regulator of chromosome condensation 1
  • regulator of chromosome condensation
  • SNHG3-RCC1 readthrough transcript
  • SNHG3-RCC1

Background

Promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensationin the S phase. Binds to the chromatin. RCC1/Ran complex (together with other proteins) acts as a component of asignal transmission pathway that detects unreplicated DNA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RCC1 Antibody (NBP1-85637) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RCC1 Antibody (NBP1-85637) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for RCC1 Antibody (NBP1-85637)

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Blogs on RCC1.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

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Bioinformatics

Gene Symbol RCC1