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NUAK2 Antibody

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Biological Strategies: Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation and activation. FBS and LPA activate YAP/TAZ and induce ...read more
Immunocytochemistry/ Immunofluorescence: NUAK2 Antibody [NBP1-81880] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Western Blot: NUAK2 Antibody [NBP1-81880] - Analysis in control (vector only transfected HEK293T lysate) and NUAK2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human cerebellum shows strong membranous positivity in Purkinje cells.
Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human duodenum shows moderate to strong nuclear positivity in glandular cells.
Genetic Strategies: Knockdown Validated: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, and target gene ...read more
Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation & activation. a, b FBS & LPA activate YAP/TAZ & induce NUAK2 expression. YAP/TAZ ...read more
Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots ...read more
Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots ...read more
Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 is associated with high-grade human bladder cancer. a, c Expression of NUAK2 & the YAP Signature Genes (YAP SG) is enhanced both in high-grade non-muscle-invasive ...read more
Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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NUAK2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUAK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Knockdown Validated
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NUAK2 Protein (NBP1-81880PEP)
Publications
Read Publications using
NBP1-81880 in the following applications:

  • WB
    3 publications

Reactivity Notes

Rat (81%). Use in Mouse reported in scientific literature (PMID:31350328).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NUAK2 Antibody

  • DKFZp434J037
  • EC 2.7.11
  • EC 2.7.11.1
  • FLJ90349
  • NUAK family SNF1-like kinase 2
  • NUAK family, SNF1-like kinase, 2
  • NUAK2
  • OMPHK2
  • SNARK
  • SNARKDKFZp686F01113
  • SNF1/AMP activated protein kinase
  • SNF1/AMP kinase-related kinase

Background

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment byincreasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cellsfrom CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NUAK2 Antibody (NBP1-81880)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NUAK2
Entrez
Uniprot