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MELK Antibody

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Western blot shows lysates of Huh-7 human hepatoma cell line, MO7e human megakaryocytic leukemic cell line, and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MELK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4820) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MELK at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Huh-7 human hepatoma cell line, MO7e human megakaryocytic leukemic cell line, and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ...read more
MELK was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human MELK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4820) at 10 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4820-SP
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MELK Antibody Summary

Immunogen
E. coli-derived recombinant human MELK
Gln341-Ala470
Accession # Q14680
Specificity
Detects human MELK in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
MELK
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using AF4820.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MELK Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • hMELK
  • HPK38
  • KIAA0175pEg3 kinase
  • maternal embryonic leucine zipper kinase
  • MELK
  • PK38
  • Protein kinase PK38

Background

MELK (maternal embryonic leucine zipper kinase; also HPK38) is a member of the Snf1/AMPK family of serine/threonine kinases. It is expressed in blood mononuclear cells, stem cells and other tissues, and functions in cell cycle progression and pre-mRNA splicing. Human MELK is 651 amino acids (aa) in length and contains one protein kinase domain (aa 11‑263) and a kinase-associated (KA) 1 domain (aa 602‑651). MELK is activated upon phosphorylation of T167 and S171. There are multiple alternative splice variants. Two show the same 16 aa substitution for the N-terminal 87 and 48 aa, respectively; a third shows an alternate start site at M440, and a fourth shows complex splicing over aa 1‑392. Over aa 341‑470, human MELK shares 56% aa identity with mouse MELK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MELK Antibody (AF4820)(1)


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Bioinformatics

Gene Symbol MELK
Uniprot