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CNAP1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CNAP1 Antibody [NBP1-88202] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Western Blot: CNAP1 Antibody [NBP1-88202] -Analysis in human cell line RH-30.
Immunohistochemistry-Paraffin: CNAP1 Antibody [NBP1-88202] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: CNAP1 Antibody [NBP1-88202] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: CNAP1 Antibody [NBP1-88202] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CNAP1 Antibody [NBP1-88202] -Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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CNAP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VGTIQCLEEILCEFVQKDELKPAVTQLLWERATEKVACCPLERCSSVMLLGMMARGKPEIVGSNLDTLVSIGLDEKFPQDYRLAQQVCHAIANISDRRKPSLGKRH
Predicted Species
Mouse (90%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCAPD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CNAP1 Protein (NBP1-88202PEP)
Publications
Read Publication using NBP1-88202.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CNAP1 Antibody

  • CAPD2
  • CAP-D2
  • Chromosome condensation-related SMC-associated protein 1
  • Chromosome-associated protein D2
  • CNAP1XCAP-D2 homolog
  • hCAP-D2condensin complex subunit 1
  • KIAA0159chromosome condensation related SMC associated protein 1
  • Non-SMC condensin I complex subunit D2
  • non-SMC condensin I complex, subunit D2

Background

CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CNAP1 Antibody (NBP1-88202)(1)

Reviews for CNAP1 Antibody (NBP1-88202) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CNAP1 Antibody (NBP1-88202) (0)

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Secondary Antibodies

 

Isotype Controls

Additional CNAP1 Products

Array NBP1-88202

Research Areas for CNAP1 Antibody (NBP1-88202)

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Blogs on CNAP1

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Read our latest blog and use the new citation tool on bio-techne.com

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Contact Information

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Bioinformatics

Gene Symbol NCAPD2