Recombinant Human Fas Ligand/TNFSF6 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a 40 kDa type II transmembrane member of the TNF superfamily of proteins. Its ability to induce apoptosis in target cells plays an important role in the development, homeostasis, and function of the immune system (1). Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain (2). Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Both mouse and human Fas Ligand are active on mouse and human cells (2, 3). Fas Ligand is expressed on the cell surface as a nondisulfide-linked homotrimer on activated CD4+ Th1 cells, CD8+ cytotoxic T cells, and NK cells (1). Fas Ligand binding to Fas/CD95 on an adjacent cell triggers apoptosis in the Fas‑expressing cell (2, 4). Fas Ligand also binds DcR3 which is a soluble decoy receptor that interferes with Fas Ligand-induced apoptosis (5). Fas Ligand can be released from the cell surface by metalloproteinases as a 26 kDa soluble molecule which remains trimeric (6, 7). Shed Fas Ligand retains the ability to bind Fas, although its ability to trigger apoptosis is dramatically reduced (6, 7). In the absence of TGF‑ beta , however, Fas Ligand/Fas interactions instead promote neutrophil-mediated inflammatory responses (3, 8). Fas Ligand itself transmits reverse signals that costimulate the proliferation of freshly antigen-stimulated T cells (9). Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites (10). This function is exploited by tumor cells which evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes (8, 11). In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity (12, 13).

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, Complex Species Category, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 3 applications: Bioassay, Surface Plasmon Resonance, Surface Plasmon Resonance (SPR.

Showing Publications 1 - 10 of 20. Show All 20 Publications. Collapse Publications.

Publications using 126-FL/CF	Applications	Species
W Zhu, H Chu, Y Zhang, T Luo, H Yu, H Zhu, Y Liu, H Gao, Y Zhao, Q Li, X Wang, G Li, W Yang Fructose-1,6-bisphosphatase 1 dephosphorylates IkappaBalpha and suppresses colorectal tumorigenesis Cell Research, 2023-01-16;0(0):. 2023-01-16 [PMID: 36646759] (Bioassay, Human)	Bioassay	Human
Bhuyan S, Pal B, Pathak L, Saikia P, Mitra S, Gayan S, Mokhtari R, Li H, Ramana C, Baishya D, Das B Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense. Front Immunol, 2022-09-29;13(0):933329. 2022-09-29 [PMID: 36248858] (Bioassay, Complex Species Category)	Bioassay	Complex Species Category
F Velard, A Chatron-Co, D Côme, MD Ah-Kioon, H Lin, N Hafsia, M Cohen-Sola, HK Ea, F Lioté Adrenomedullin and truncated peptide adrenomedullin(22-52) affect chondrocyte response to apoptotis in vitro: downregulation of FAS protects chondrocyte from cell death Sci Rep, 2020-10-07;10(1):16740. 2020-10-07 [PMID: 33028903] (Bioassay, Bovine)	Bioassay	Bovine
AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 2018-03-05;0(0):1-43. 2018-03-05 [PMID: 29436901] (Surface Plasmon Resonance (SPR, Human)	Surface Plasmon Resonance (SPR	Human
M Frank-Bert, DS Pisetsky, C Kolling, BA Michel, RE Gay, A Jüngel, S Gay TLR3 Ligand Poly(I:C) Exerts Distinct Actions in Synovial Fibroblasts When Delivered by Extracellular Vesicles Front Immunol, 2018-01-29;9(0):28. 2018-01-29 [PMID: 29434584] (Bioassay, Human)	Bioassay	Human
N Han, L Shi, Q Guo, W Sun, Y Yu, L Yang, X Zhang, M Zhang HAT1 induces lung cancer cell apoptosis via up regulating Fas Oncotarget, 2017-09-23;8(52):89970-89977. 2017-09-23 [PMID: 29163803] (Bioassay, Human)	Bioassay	Human
Bo Ra You, Bo Ram Han, Woo Hyun Park Suberoylanilide hydroxamic acid increases anti-cancer effect of tumor necrosis factor-? through up-regulation of TNF receptor 1 in lung cancer cells. Oncotarget, 2017-03-14;0(0):1949-2553. 2017-03-14 [PMID: 28099148] (Bioassay, Human)	Bioassay	Human
Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis Sci Rep, 2016-11-18;6(0):36862. 2016-11-18 [PMID: 27857173] (Bioassay, Human)	Bioassay	Human
Cui X, Zhang L, Magli A, Catera R, Yan X, Griffin D, Rothstein T, Barrientos J, Kolitz J, Allen S, Rai K, Chiorazzi N, Chu C Cytoplasmic myosin-exposed apoptotic cells appear with caspase-3 activation and enhance CLL cell viability. Leukemia, 2015-07-29;30(1):74-85. 2015-07-29 [PMID: 26220042] (Bioassay, Human)	Bioassay	Human
Liang D, Hou Y, Lou X, Chen H Decoy Receptor 3 Improves Survival in Experimental Sepsis by Suppressing the Inflammatory Response and Lymphocyte Apoptosis. PLoS ONE, 2015-06-29;10(6):e0131680. 2015-06-29 [PMID: 26121476] (Bioassay, Human)	Bioassay	Human
Li B, Baylink D, Walter M, Lau K, Meng X, Wang J, Cherkas A, Tang X, Qin X Targeted 25-hydroxyvitamin D3 1alpha-hydroxylase adoptive gene therapy ameliorates dss-induced colitis without causing hypercalcemia in mice. Mol Ther, 2014-10-20;23(2):339-51. 2014-10-20 [PMID: 25327179] (Bioassay, Mouse)	Bioassay	Mouse
You B, Park W Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels. Int J Oncol, 2014-03-12;44(5):1745-55. 2014-03-12 [PMID: 24626405] (Bioassay, Human)	Bioassay	Human
Takahashi Y, Byrareddy S, Albrecht C, Brameier M, Walter L, Mayne A, Dunbar P, Russo R, Little D, Villinger T, Khowawisetsut L, Pattanapanyasat K, Villinger F, Ansari A In vivo administration of a JAK3 inhibitor during acute SIV infection leads to significant increases in viral load during chronic infection. PLoS Pathog, 2014-03-06;10(3):e1003929. 2014-03-06 [PMID: 24603870] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque))	Bioassay	Primate - Macaca mulatta (Rhesus Macaque)
Kyathanahalli , Chandras, Marks , Jason, Nye , Kennedy, Lao , Belinda, Albrecht , Eugene D, Aberdeen , Graham W, Nathanielsz , Peter W, Jeyasuria , Panchara, Condon , Jennifer Cross-species withdrawal of MCL1 facilitates postpartum uterine involution in both the mouse and baboon. Endocrinology, 2013-10-18;154(12):4873-84. 2013-10-18 [PMID: 24140717] (Bioassay, Human)	Bioassay	Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)	Bioassay	Human
Sagiv , A, Biran , A, Yon , M, Simon , J, Lowe , S W, Krizhanovsky , V Granule exocytosis mediates immune surveillance of senescent cells. Oncogene, 2012-07-02;32(15):1971-7. 2012-07-02 [PMID: 22751116] (Bioassay, Human)	Bioassay	Human
Stephenson-Famy A, Marks J, Suresh A Antiapoptotic signaling via MCL1 confers resistance to caspase-3-mediated apoptotic cell death in the pregnant human uterine myocyte. Mol. Endocrinol., 2011-12-22;26(2):320-30. 2011-12-22 [PMID: 22194343] (Bioassay, Human)	Bioassay	Human
Lobito AA, Ramani SR, Tom I, Bazan JF, Luis E, Fairbrother WJ, Ouyang W, Gonzalez LC Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1. J. Biol. Chem., 2011-03-31;286(21):18969-81. 2011-03-31 [PMID: 21454693] (Surface Plasmon Resonance, Human)	Surface Plasmon Resonance	Human
Titanji K, Velu V, Chennareddi L Acute depletion of activated memory B cells involves the PD-1 pathway in rapidly progressing SIV-infected macaques. J. Clin. Invest., 2010-10-25;120(11):3878-90. 2010-10-25 [PMID: 20972331] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque))	Bioassay	Primate - Macaca mulatta (Rhesus Macaque)
De Milito A, Iessi E, Logozzi M, Lozupone F, Spada M, Marino ML, Federici C, Perdicchio M, Matarrese P, Lugini L, Nilsson A, Fais S Proton pump inhibitors induce apoptosis of human B-cell tumors through a caspase-independent mechanism involving reactive oxygen species. Cancer Res., 2007-06-01;67(11):5408-17. 2007-06-01 [PMID: 17545622] (Bioassay, Human)	Bioassay	Human
Show All 20 Publications. Collapse Publications.

Array 126-FL/CF

• Fas Ligand/TNFSF6 Antibodies
• Fas Ligand/TNFSF6 ELISA Kits
• Fas Ligand/TNFSF6 Lysate
• Fas Ligand/TNFSF6 Protein
• Fas Ligand/TNFSF6 Proteins and Enzymes
• Fas Ligand/TNFSF6 ELISAs