NUAK2 Antibodies
We offer NUAK2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each NUAK2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NUAK2 Antibodies can be used in a variety of model species: Human. Use the list below to choose the NUAK2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NUAK2 polyclonal antibodies and browse our NUAK2 monoclonal antibody catalog.
Alternate Names for NUAK2 Antibodies
anti-NUAK2 antibody, anti-DKFZp434J037 antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.1 antibody, anti-FLJ90349 antibody, anti-NUAK family SNF1-like kinase 2 antibody, anti-NUAK family, SNF1-like kinase, 2 antibody, anti-SNARKDKFZp686F01113 antibody, anti-SNF1/AMP activated protein kinase antibody, anti-SNF1/AMP kinase-related kinase antibody
5 Results for "nuak2" in Primary Antibodies
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation and activation. FBS and LPA activate YAP/TAZ and induce NUAK2 expression. YAP/TAZ phosphorylation status was monitored using Phos-Tag gels, while NUAK2 and ANKRD1 mRNA expression was determined by qPCR at the indicated times. Expression data are plotted as the mean +/- SD (n = 3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05939-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Western-Blot-NBP1-81880-img0017.jpg "<b>Biological Strategies Validation. </b>Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation and activation. FBS and LPA activate YAP/TAZ and induce NUAK2 expression. YAP/TAZ phosphorylation status was monitored using Phos-Tag gels, while NUAK2 and ANKRD1 mRNA expression was determined by qPCR at the indicated times. Expression data are plotted as the mean +/- SD (n = 3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05939-2), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NUAK2 Antibody [NBP1-81880] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81880-img0010.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0015.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - Analysis in control (vector only transfected HEK293T lysate) and NUAK2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NUAK2-Antibody-Western-Blot-NBP1-81880-img0011.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human cerebellum shows strong membranous positivity in Purkinje cells.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0012.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0013.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human duodenum shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, and target gene expression in MDA-MB231 cells. YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05939-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Knockdown-Validated-NBP1-81880-img0016.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation & activation. a, b FBS & LPA activate YAP/TAZ & induce NUAK2 expression. YAP/TAZ phosphorylation status was monitored using Phos-Tag gels (a), while NUAK2 & ANKRD1 mRNA expression was determined by qPCR at the indicated times. Expression data are plotted as the mean ± SD (n = 3). c–h WZ4003 (10 μM) blocks serum/LPA-induced NUAK2 & ANKRD1 mRNA expression. FBS-induced or LPA-induced YAP/TAZ dephosphorylation (e & g), or NUAK2 & ANKRD1 mRNA expression (c, f, h) was monitored at the indicated times (d). Expression data are plotted as the mean ± range for a representative experiment (c, f, h) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-31020241535685.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-31020241612198.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-310202416113541.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 is associated with high-grade human bladder cancer. a, c Expression of NUAK2 & the YAP Signature Genes (YAP SG) is enhanced both in high-grade non-muscle-invasive (HG-NMIBC; n = 13, **p = 0.0071) & muscle-invasive (pT2 MIBC; n = 9, **p = 0.0033) bladder cancers39 as compared to LG-NMIBC (n = 27) using an unpaired t test. b Elevated expression of NUAK2 is correlated with disease recurrence in a cohort41 of MIBC patient samples with n = 57 disease free & n = 56, recurred samples (*p = 0.0172). Box & whisker plots show the median (line in the box), first & third quartiles (lower & upper ends of the box), & the minimum & maximum values (whiskers in the plot). Dots represent a single patient sample. d A heat map depicting expression of NUAK2 & YAP SG in NMIBC LG (open circle), HG (closed circle), & MIBC pT2 (slashed circle) bladder cancer samples39. e Characterization of high-grade-derived & low-grade-derived bladder cancer cell lines. Scale bar, 25 μm. f NUAK2 mRNA expression is elevated in HG-derived (TCCSUP & T24) BC cell lines as compared to LG lines. Data are plotted as the mean ± SD (n = 3). g–i Blocking NUAK2 activity with 10 μM WZ4003 (g) or NUAK2 expression in stable clones (#5 or #7) using inducible NUAK2 shRNAs (h, i) inhibits the growth of HG BC cell lines as measured by SRB (g) or DAPI (h) staining. Data are plotted as the mean ± SD of a representative experiment, n = 3 (for TCC) or mean ± range of two experiments (for T24) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-310202416114727.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-31020241612229.jpg)
![Western Blot: NUAK2 Antibody (2F9) [H00081788-M04] - Analysis of NUAK2 expression in transfected 293T cell line by NUAK2 monoclonal antibody (M04), clone 2F9. Lane 1: NUAK2 transfected lysate (Predicted MW: 69.6 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NUAK2-Antibody-2F9-Western-Blot-H00081788-M04-img0003.jpg "Western Blot: NUAK2 Antibody (2F9) [H00081788-M04] - Analysis of NUAK2 expression in transfected 293T cell line by NUAK2 monoclonal antibody (M04), clone 2F9. Lane 1: NUAK2 transfected lysate (Predicted MW: 69.6 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NUAK2 Antibody (2F9) [H00081788-M04] - Analysis of monoclonal antibody to NUAK2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/NUAK2-Antibody-2F9-Immunocytochemistry-Immunofluorescence-H00081788-M04-img0001.jpg)
![Sandwich ELISA: NUAK2 Antibody (2F9) [H00081788-M04] - Detection limit for recombinant GST tagged NUAK2 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NUAK2-Antibody-2F9-Sandwich-ELISA-H00081788-M04-img0002.jpg)
![Western Blot: NUAK2 Antibody [NBP2-19599] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Western-Blot-NBP2-19599-img0001.jpg "Western Blot: NUAK2 Antibody [NBP2-19599] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP2-19599] - Human gastric cancer, using NUAK2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP2-19599-img0002.jpg)
![Western Blot: NUAK2 Antibody [NBP2-94277] - Analysis of extracts of rat liver, using NUAK2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Western-Blot-NBP2-94277-img0001.jpg "Western Blot: NUAK2 Antibody [NBP2-94277] - Analysis of extracts of rat liver, using NUAK2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Western Blot: NUAK2 Antibody [H00081788-B01P] - Analysis of NUAK2 expression in transfected 293T cell line by NUAK2 polyclonal antibody. Lane 1: NUAK2 transfected lysate(69.08 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Western-Blot-H00081788-B01P-img0002.jpg "Western Blot: NUAK2 Antibody [H00081788-B01P] - Analysis of NUAK2 expression in transfected 293T cell line by NUAK2 polyclonal antibody. Lane 1: NUAK2 transfected lysate(69.08 KDa). Lane 2: Non-transfected lysate.")