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Rat NT-3 ELISA Kit (Colorimetric)

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ELISA: Rat NT-3 ELISA Kit (Colorimetric) [NBP2-76768] - Samples were spiked with high concentrations of Rat NT-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat NT-3 ELISA Kit (Colorimetric) [NBP2-76768] - Samples were spiked with high concentrations of Rat NT-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat NT-3 ELISA Kit (Colorimetric) [NBP2-76768] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.156 - 10ng/mL
Sensitivity
0.094ng/mL

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Rat NT-3 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.156 - 10ng/mL
Sensitivity
0.094ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5
Intra-Assay
CV% < 4.61
Spike Recovery
86-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
NTF3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat NT-3 ELISA Kit (Colorimetric)

  • HDNF
  • MGC129711
  • Nerve growth factor 2
  • Neurotrophic factor
  • neurotrophin 3
  • neurotrophin-3
  • NGF2
  • NGF-2
  • NT3
  • NT-3
  • NTF3

Background

NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. Subcellular Location: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. Similarity: Belongs to the NGF-beta family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Product PDFs

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  Rat NT-3 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol NTF3