![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining in human kidney and pancreas tissues using NBP1-82559 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0014.jpg "Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining in human kidney and pancreas tissues using NBP1-82559 antibody. Corresponding SLC12A1 RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: SQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDD |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC12A1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Immunohistochemistry Neurophysin II/Arg-vasopressin Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af009_human-neurophysin-ii-affinity-purified-polyclonal-ab-immunohistochemistry-2812202013195.jpg)
| Publication using NBP1-82559 | Applications | Species |
|---|---|---|
| Harari-Steinberg O, Omer D, Gnatek Y et al. Ex Vivo Expanded 3D Human Kidney Spheres Engraft Long Term and Repair Chronic Renal Injury in Mice Cell Rep 2020-01-21 [PMID: 31968258] (IHC-P, Mouse) | IHC-P | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for NKCC2/SLC12A1 Antibody (NBP1-82559)Find related products by research area.
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| Gene Symbol | SLC12A1 |
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0009.jpg "Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.")
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human cerebellum, kidney, liver and pancreas using Anti-SLC12A1 antibody NBP1-82559 (A) shows similar protein distribution across tissues to independent antibody NBP1-80993 (B).](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0010.jpg "Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human cerebellum, kidney, liver and pancreas using Anti-SLC12A1 antibody NBP1-82559 (A) shows similar protein distribution across tissues to independent antibody NBP1-80993 (B).")
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0011.jpg "Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human kidney shows strong membranous positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0012.jpg "Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/NKCC2-SLC12A1-Antibody-Immunohistochemistry-Paraffin-NBP1-82559-img0013.jpg "Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP1-82559] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")