![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Pax8-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules-AQP1, distal tubules-THP and collecting ducts-AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aquaporin-1-AQP1-Antibody-1-A5F6-Immunohistochemistry-NB600-749-img0002.jpg "Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Pax8-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules-AQP1, distal tubules-THP and collecting ducts-AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY license.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 1/A5F6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | Synthetic peptide corresponding to amino acids 249-269 of aquaporin 1 |
| Localization | Plasma membrane |
| Specificity | Antibody recognises an epitope within the cytoplasmic domain of the water-specific channel aquaporin 1. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AQP1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | ELISA: Use at a dilution of 1/1000. IHC-P: Use at a dilution of 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.IHC-Fr: Use at a dilution of 1/500. IP: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Predicted molecular weight: 32.3 kDa. Immunofluorescence staining reported successful by customer review. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
![Immunohistochemistry Neurophysin II/Arg-vasopressin Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af009_human-neurophysin-ii-affinity-purified-polyclonal-ab-immunohistochemistry-2812202013195.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IF | Rat | 11/30/2010 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Aquaporin 1/AQP1 Antibody (NB600-749)Find related products by research area.
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![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localized to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/srep11061), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aquaporin-1-AQP1-Antibody-1-A5F6-Immunohistochemistry-NB600-749-img0001.jpg "Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localized to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/srep11061), licensed under a CC-BY license.")
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Slc22a6-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice (a); kidneys from vehicle treated mice were negative (b). Co-localisation studies revealed specific X-gal expression within proximal tubular epithelium (AQP1) with no expression detected in distal tubules (THP) or collecting ducts (AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aquaporin-1-AQP1-Antibody-1-A5F6-Immunohistochemistry-NB600-749-img0003.jpg "Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Slc22a6-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice (a); kidneys from vehicle treated mice were negative (b). Co-localisation studies revealed specific X-gal expression within proximal tubular epithelium (AQP1) with no expression detected in distal tubules (THP) or collecting ducts (AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys & urinary bladder.(a) Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies & merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) & distal (THP) renal tubular epithelial cells & renal fibroblasts (CD73) (white arrowheads). Strawberry was not localised to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 μm. (b) Confocal images of Strawberry expression in the liver, spleen, lung, urinary bladder & left & right kidney of ELS infected animals 60 days post left intrarenal injection (top panels) & uninjected control animals (bottom panels). Sections were stained with an antibody against Strawberry (green) & merged images with DAPI (blue) are presented. Scale bars, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26046460), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-749_mouse-monoclonal-aquaporin-1-aqp1-antibody-1-a5f6-31020241617140.jpg "Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys & urinary bladder.(a) Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies & merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) & distal (THP) renal tubular epithelial cells & renal fibroblasts (CD73) (white arrowheads). Strawberry was not localised to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 μm. (b) Confocal images of Strawberry expression in the liver, spleen, lung, urinary bladder & left & right kidney of ELS infected animals 60 days post left intrarenal injection (top panels) & uninjected control animals (bottom panels). Sections were stained with an antibody against Strawberry (green) & merged images with DAPI (blue) are presented. Scale bars, 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26046460), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - USSC administration recovered aquaporin1 (AQP1) expression in the choroid plexus after PHH. (A) Representative immunofluorescence image of cryosections labeled with AQP1 specific antibody (arrows showing choroid epithelium immunoreactivity) on postnatal days 7. Strong AQP1 immunoreactivity in the control (blue arrows), reduced signal (yellow arrows) in IVH followed by recovered expression (blue arrows), & images from left to right respectively at postnatal day 7. CPE (Choroid plexus epithelial cells). Images were taken at 20× objective; blue = DAPI stain, 20 μm sections. Scale bar 100 μm. (B) Strong AQP1 immunoreactivity in the control (blue arrows), reduced signal (yellow arrows) in IVH followed by recovered expression (blue arrows), & the images from left to right respectively at day 14. Images were taken at 20× objective; blue = DAPI stain, 20 μm sections. Scale bar 100 μm. (C) Representative immunofluorescence image of cryosections labeled with aquaporin1. As seen in the high power image (panel-B above), the strong AQP1 immunoreactivity is evident over the entire choroid plexus in the control, reduced signal in IVH followed by recovered strong AQP1 expression observed in images from left to right respectively at day 14. Images were taken at 4× objective; 20 μm sections. The scale bar is 500 μm. Fluorescence images were taken using “Nikon Eclipse 90i microscope” (Nikon Instruments, Japan). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33897371), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-749_mouse-monoclonal-aquaporin-1-aqp1-antibody-1-a5f6-310202416171417.jpg "Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - USSC administration recovered aquaporin1 (AQP1) expression in the choroid plexus after PHH. (A) Representative immunofluorescence image of cryosections labeled with AQP1 specific antibody (arrows showing choroid epithelium immunoreactivity) on postnatal days 7. Strong AQP1 immunoreactivity in the control (blue arrows), reduced signal (yellow arrows) in IVH followed by recovered expression (blue arrows), & images from left to right respectively at postnatal day 7. CPE (Choroid plexus epithelial cells). Images were taken at 20× objective; blue = DAPI stain, 20 μm sections. Scale bar 100 μm. (B) Strong AQP1 immunoreactivity in the control (blue arrows), reduced signal (yellow arrows) in IVH followed by recovered expression (blue arrows), & the images from left to right respectively at day 14. Images were taken at 20× objective; blue = DAPI stain, 20 μm sections. Scale bar 100 μm. (C) Representative immunofluorescence image of cryosections labeled with aquaporin1. As seen in the high power image (panel-B above), the strong AQP1 immunoreactivity is evident over the entire choroid plexus in the control, reduced signal in IVH followed by recovered strong AQP1 expression observed in images from left to right respectively at day 14. Images were taken at 4× objective; 20 μm sections. The scale bar is 500 μm. Fluorescence images were taken using “Nikon Eclipse 90i microscope” (Nikon Instruments, Japan). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33897371), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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