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VASA Antibody [Unconjugated]

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Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG ...read more
VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before incubation with ...read more
Immuno-staining on control and PMSG treated ovarian sections.A-C: Immunostaining with anti-PCNA antibody. Note minimal PCNA staining in control OSE. Increased staining is observed after PMSG treatment in both the OSE ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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VASA Antibody [Unconjugated] Summary

Specificity
Detects human VASA in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DDX4
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2030 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VASA Antibody [Unconjugated]

  • DDX4
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • DEAD box protein 4
  • EC 3.6.1
  • EC 3.6.4.13
  • MGC111074
  • probable ATP-dependent RNA helicase DDX4
  • Vasa homolog
  • VASA
  • VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is therefore a highly specific marker of germ cells (1-3).
  1. Zeeman, A.M. et al. (2002) Lab Invest. 82:159.
  2. Raz, E. (2000) Genome Biol. 1:REVIEWS1017.
  3. Castrillon, D. et al. (2001) Proc. Natl. Acad. Sci. USA 97:9585.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VASA Antibody (AF2030)(56)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 8 applications: Flow Cytometry, ICC, ICC/IF, IF, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(4)
ICC
(11)
ICC/IF
(1)
IF
(1)
IHC
(9)
IHC-Fr
(1)
IHC-P
(6)
Western Blot
(1)
All Applications
Filter By Species
Human
(17)
Mouse
(9)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 56. Show All 56 Publications.
Publications using AF2030 Applications Species
Penny A. F. Whiley, Michael C. M. Luu, Liza O'Donnell, David J. Handelsman, Kate L. Loveland Testis exposure to unopposed/elevated activin A in utero affects somatic and germ cells and alters steroid levels mimicking phthalate exposure Front Endocrinol (Lausanne) 2023-09-01 [PMID: 37727456]
M. Bashawat, B.C. Braun, K. Müller, B.P. Hermann Molecular phenotyping of domestic cat (Felis catus) testicular cells across postnatal development – A model for wild felids Theriogenology Wild 2023-07-18 [PMID: 37461433]
Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm et al. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay Cell Reports Methods 2023-06-26 [PMID: 37426764]
Zhang, X;Liu, Y;Sosa, F;Gunewardena, S;Crawford, PA;Zielen, AC;Orwig, KE;Wang, N; Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells Cell reports 2023-07-04 [PMID: 37405912] (ICC/IF, Western Blot, IHC, Mouse) ICC/IF, Western Blot, IHC Mouse
AS Bailey, MT Fuller YTHDC2 serves a distinct late role in spermatocytes during germ cell differentiation bioRxiv : the preprint server for biology, 2023-01-23;0(0):. 2023-01-23 [PMID: 36747642] (IHC, Mouse) IHC Mouse
Yasunari Seita, Keren Cheng, John R McCarrey, Nomesh Yadu, Ian H Cheeseman, Alec Bagwell et al. Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells eLife 2023-01-31 [PMID: 36719274]
Zhao S, Huang C, Yang Y et al. DNA repair protein FANCD2 has both ubiquitination-dependent and ubiquitination-independent functions during germ cell development Journal of Biological Chemistry 2023-03-01 [PMID: 36642183]
T Lehtiniemi, M Bourgery, L Ma, A Ahmedani, M Mäkelä, J Asteljoki, O Olotu, S Laasanen, FP Zhang, K Tan, JN Chousal, D Burow, S Koskinen, A Laiho, LL Elo, F Chalmel, MF Wilkinson, N Kotaja SMG6 localizes to the chromatoid body and shapes the male germ cell transcriptome to drive spermatogenesis Nucleic Acids Research, 2022-11-11;0(0):. 2022-11-11 [PMID: 36259644] (IHC, Human) IHC Human
Sarah C. Moody, Penny A. F. Whiley, Patrick S. Western, Kate L. Loveland The Impact of Activin A on Fetal Gonocytes: Chronic Versus Acute Exposure Outcomes Front Endocrinol (Lausanne) 2022-05-31 [PMID: 35721752]
Xin Mi, Wenlin Jiao, Yajuan Yang, Yingying Qin, Zi-Jiang Chen, Shidou Zhao HGF Secreted by Mesenchymal Stromal Cells Promotes Primordial Follicle Activation by Increasing the Activity of the PI3K-AKT Signaling Pathway Stem Cell Reviews and Reports 2022-06-01 [PMID: 35089464]
Show All 56 Publications.

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Bioinformatics

Gene Symbol DDX4
Uniprot