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VASA Antibodies

We offer VASA Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each VASA Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our VASA Antibodies can be used in a variety of model species: Canine, Equine, Human, Mouse, Primate, Rat. Use the list below to choose the VASA Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our VASA polyclonal antibodies and browse our VASA monoclonal antibody catalog.

Alternate Names for VASA Antibodies

anti-VASA antibody, anti-DDX4 antibody, anti-DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 antibody, anti-DEAD box protein 4 antibody, anti-EC 3.6.1 antibody, anti-EC 3.6.4.13 antibody, anti-MGC111074 antibody, anti-probable ATP-dependent RNA helicase DDX4 antibody, anti-Vasa homolog antibody, anti-VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody

10 Results for "vasa" in Primary Antibodies

AF2030

VASA Antibody [Unconjugated]

(56 Publications)

100 μg

AF2030

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immuno-staining on control and PMSG treated ovarian sections.A-C: Immunostaining with anti-PCNA antibody. Note minimal PCNA staining in control OSE. Increased staining is observed after PMSG treatment in both the OSE cells and the oocytes of PF (B-&C). Inset B’ is magnified image of the PF located in the OSE. At places, granulosa cells were also positive for PCNA (arrowhead). D-F: Immunostaining with anti-OCT-4 antibody in control (D) and 7D PMSG treated (E&F) ovarian sections. OCT-4 is localized in the ooplasm in control, while in 7D PMSD treated, positive staining was observed in ooplasm as well as in the nucleus of few oocytes (arrowhead) in PFs. G: Immunostaining with anti-human VASA antibody that cross-reacts with mouse MVH. MVH is distinctly localized in the ooplasm of PF and at places was also observed in a ‘germ cell nest’ (G’, arrow). At places some oocytes of primordial follicles in cohorts appeared connected without intervening granulosa cell (arrowhead) H-J: SCP-3 is localized in PF oocytes present in close vicinity of multilayer OSE. Bar: 20μm. Image collected and cropped by CiteAb from the following publication (//ovarianresearch.biomedcentral.com/articles/10.1186/1757-2215-5-32), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2030

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2030-SP

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NBP2-24558

VASA Antibody

(7 Publications)

0.1 mg

NBP2-24558

Immunohistochemistry-Paraffin: VASA Antibody [NBP2-24558] - Analysis of DDX4 in mouse testis tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Eq, Pm, Xp, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Pm, Xp, Ze

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6480A

Alt Catalog# IMG-6480A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-20844

VASA Antibody

0.1 ml

NBP2-20844

Western Blot: VASA Antibody [NBP2-20844] - Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: VASA Antibody [NBP2-20844] - Paraffin-embedded mouse testis. DDX4 antibody [C1C3] diluted at 1:500.

Western Blot: VASA Antibody [NBP2-20844] - Mouse tissue extract (30 | 50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VASA antibody [C1C3] (NBP2-20844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: VASA Antibody [NBP2-20844] - Human tissue extracts (30 | 50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VASA antibody [C1C3] (NBP2-20844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Fi, Rt, Po, Bv, Pm, RM

Species Glossary

Reactivity: Hu, Mu, Fi, Rt, Po, Bv, Pm, RM

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-25431

VASA Antibody (HL1487) - Azide and BSA Free

100 ul

NBP3-25431

Immunohistochemistry-Paraffin: VASA Antibody (HL1487) - Azide and BSA Free [NBP3-25431] - DDX4 antibody [HL1487] detects DDX4 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. DDX4 stained by DDX4 antibody [HL1487] (NBP3-25431) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: VASA Antibody (HL1487) - Azide and BSA Free [NBP3-25431] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (NBP3-25431) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: VASA Antibody (HL1487) - Azide and BSA Free [NBP3-25431] - Human tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (NBP3-25431) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: VASA Antibody (HL1487) - Azide and BSA Free [NBP3-25431] - DDX4 antibody [HL1487] detects DDX4 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. DDX4 stained by DDX4 antibody [HL1487] (NBP3-25431) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: VASA Antibody (HL1487) - Azide and BSA Free [NBP3-25431] - DDX4 antibody [HL1487] detects DDX4 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DDX4 stained by DDX4 antibody [HL1487] (NBP3-25431) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: VASA Antibody (HL1487) - Azide and BSA Free [NBP3-25431] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [HL1487] (NBP3-25431) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP1-51586

VASA Antibody (2F9H5) - BSA Free

0.1 ml

NBP1-51586

Western Blot: VASA Antibody (2F9H5) [NBP1-51586] - Analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.

Immunocytochemistry/Immunofluorescence: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Immunohistochemistry-Paraffin: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.

Flow Cytometry: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-51586-0.025ml

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MAB2030

VASA Antibody (246904) [Unconjugated]

(1 Publication)

500 μg

MAB2030

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VASA Monoclonal Antibody (Catalog # MAB2030) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2030-SP

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NBP2-13907

VASA Antibody

0.1 ml

NBP2-13907

Immunohistochemistry-Paraffin: VASA Antibody [NBP2-13907] - Staining of human testis shows high expression.

Immunohistochemistry-Paraffin: VASA Antibody [NBP2-13907] - Staining of human endometrium shows low expression as expected.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VASA Antibody [NBP2-13907] - Staining in human testis and endometrium tissues using anti-DDX4 antibody. Corresponding DDX4 RNA-seq data are presented for the same tissues.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-13908

VASA Antibody

0.1 ml

NBP2-13908

Immunohistochemistry-Paraffin: VASA Antibody [NBP2-13908] - Staining in human testis and endometrium tissues using anti-DDX4 antibody. Corresponding DDX4 RNA-seq data are presented for the same tissues.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VASA Antibody [NBP2-13908] - Staining of human endometrium shows low expression as expected.

Immunohistochemistry-Paraffin: VASA Antibody [NBP2-13908] - Staining of human testis shows high expression.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-95280

VASA Antibody

100 ul

NBP2-95280

Immunohistochemistry-Paraffin: VASA Antibody [NBP2-95280] - Immunochemical staining VASA in human testis with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: VASA Antibody [NBP2-95280] - Staining DDX4 in human testis with rabbit polyclonal antibody (1:2000).

Reactivity:

Hu, Rt, Mu

Species Glossary

Reactivity: Hu, Rt, Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-95280AF350
Alexa Fluor 405	NBP2-95280AF405
Alexa Fluor 488	NBP2-95280AF488
Alexa Fluor 532	NBP2-95280AF532
Alexa Fluor 594	NBP2-95280AF594
Alexa Fluor 647	NBP2-95280AF647
Alexa Fluor 700	NBP2-95280AF700
Alexa Fluor 750	NBP2-95280AF750
Allophycocyanin	NBP2-95280APC
Biotin	NBP2-95280B
CoraFluor 1	NBP2-95280CL1
DyLight 350	NBP2-95280UV
DyLight 405	NBP2-95280V
DyLight 488	NBP2-95280G
DyLight 550	NBP2-95280R
DyLight 594	NBP2-95280DL594
DyLight 650	NBP2-95280C
DyLight 680	NBP2-95280FR
DyLight 755	NBP2-95280IR
FITC	NBP2-95280F
HRP	NBP2-95280H
Janelia Fluor 525	NBP2-95280JF525
Janelia Fluor 549	NBP2-95280JF549
Janelia Fluor 585	NBP2-95280JF585
Janelia Fluor 635	NBP2-95280JF635
Janelia Fluor 646	NBP2-95280JF646
Janelia Fluor 669	NBP2-95280JF669
PE	NBP2-95280PE
PerCP	NBP2-95280PCP
mFluor Violet 450 SE	NBP2-95280MFV450
mFluor Violet 500 SE	NBP2-95280MFV500
mFluor Violet 610 SE	NBP2-95280MFV610

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NBP3-30963

VASA Antibody

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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