I am from China and I would like to know more about your product number：NBL1-07638. We know that the theoretical MW of AQP3 protein is 31KD. But in your product specification, we can see that so many bands appear after overexpressing AQP3 with plasmid in 293 cell. Can you explain this phenomenon? Apart from theoretical strip, are all other nonspecific bands? Or is the AQP3 itself ?

Name: Aquaporin-3 Antibody
Brand: Novus Biologicals
SKU: NBP1-97927
Price: 449 USD

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Yes, the theoretical size of AQP3 is 31 kDa, which would be expected under reduced conditions with a sample. We have not characterized any of the other bands in the overexpressed lysate lane. Because this is a native lysate, any modifications that may take place could cause smearing and extra bands in the lysate sample. AQP3 is extensively modified because it is a glycoprotein, glycosylation often shows up as multiple bands with smearing when run under native conditions.  This overexpression lysate is meant to only act as a positive control in WB experiments requiring a native control. Furthermore, the lane on the right side of the blot is showing detection of the lysate using an anti-Flag tag antibody, since the construct used for overexpression contains a Flag tag.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	1 mg/ml

Immunogen	Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3
Localization	Membrane
Specificity	Detects approx 31.5kDa. May detect larger glycosylated bands approx 35-50kDa.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	AQP3
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS, 50% Glycerol
Preservative	0.09% Sodium Azide
Concentration	1 mg/ml
Purity	Protein A purified

Publications using NBP1-97927	Applications	Species
de Boer, LL;Vanes, L;Melgrati, S;Biggs O'May, J;Hayward, D;Driscoll, PC;Day, J;Griffiths, A;Magueta, R;Morrell, A;MacRae, JI;Köchl, R;Tybulewicz, VLJ; T cell migration requires ion and water influx to regulate actin polymerization Nature communications 2023-12-06 [PMID: 38057317]
Grabowska M, Michalek K, Kedzierska-Kapuza K et al. The long-term effects of rapamycin-based immunosuppressive protocols on the expression of renal aquaporins 1, 2, 3 and 4 water channels in rats Histology and histopathology 2021-02-26 [PMID: 33634832] (IHC-P, Rat)	IHC-P	Rat
Bollag WB, Aitkens L, White J, Hyndman KA Aquaporin-3 in the Epidermis: More than Skin Deep Am. J. Physiol., Cell Physiol. 2020-04-08 [PMID: 32267715] (IF/IHC)	IF/IHC
Gonen T, Walz T. The structure of aquaporins. Q Rev Biophys. 2006-11-01 [PMID: 17156589]
Dibas AI, Mia AJ, Yorio T et al. Aquaporins (water channels): role in vasopressin-activated water transport. Proc Soc Exp Biol Med. 1998-12-01 [PMID: 9824541]
Sasaki S, Ishibashi K, Marumo F et al. Aquaporin-2 and -3: representatives of two subgroups of the aquaporin family colocalized in the kidney collecting duct. Annu Rev Physiol. 1998-01-01 [PMID: 9558461]
Knepper MA. The aquaporin family of molecular water channels. Proc Natl Acad Sci U S A. 1994-07-01 [PMID: 7517546]

Aquaporin-3 Antibody

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Gene Symbol	AQP3
Entrez	65133(Rat)
Uniprot	P47862(Rat)