Novus Biologicals products are now on bio-techne.com

NHE3/SLC9A3 Antibody

Images

 
There are currently no images for NHE3/SLC9A3 Antibody (NBP1-46581).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

NHE3/SLC9A3 Antibody Summary

Immunogen
A synthetic peptide from amino acid region 650-700 of human NHE3/SLC9A3 conjugated to blue carrier protein was used as the antigen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC9A3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:200-1:2000
  • Immunohistochemistry-Frozen 1:200-1:2000
  • Western Blot 1:200-1:2000
Application Notes
The optimal dilution should be determined by the end user. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin. ICC/IF, and IHC-FR were reported in scientific literature (PMID: 23548792).
Publications
Read Publications using
NBP1-46581 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for NHE3/SLC9A3 Antibody

  • isoform 3
  • MGC126718
  • NHE3
  • NHE3MGC126720
  • SLC9A3
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3
  • Solute carrier family 9 member 3

Background

NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-38236
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-76847
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP3-35065
Species: Hu, Mu, Rt
Applications: ELISA, WB
NBP1-44270
Species: Ca, Hu, Mu, Rt
Applications: EM, ICC/IF, IHC, IHC-P, WB
NB100-2450
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP1-80993
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-38280
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-32020
Species: Hu
Applications: IHC, IHC-P
NBP1-30027
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-84450
Species: Hu, Mu
Applications: IHC-WhMt, IHC, IHC-P, WB
MAB7665
Species: Hu
Applications: IHC, WB
NB110-74682
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-16396
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC-WhMt, IHC, IHC-P, WB
NBP2-82026
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF1180
Species: Hu
Applications: ICC, IHC, Simple Western, WB
NBP2-20338
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-46581
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for NHE3/SLC9A3 Antibody (NBP1-46581)(6)

Reviews for NHE3/SLC9A3 Antibody (NBP1-46581) (0)

There are no reviews for NHE3/SLC9A3 Antibody (NBP1-46581). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NHE3/SLC9A3 Antibody (NBP1-46581). (Showing 1 - 1 of 1 FAQs).

  1. The molecular weight of NHE3 is 92kDa but I see a band above 100 kDa.  I am confused, because the size of these protein should not be greater than 100KD. Can you please help explain?  
    • Higher than predicted molecular weight seen on WB is not uncommon if the protein is posttranslationally modified, especially glycosylated, and such is the case for NHE3. Please note that NHE3 gets phosphorylated and glycosylated (Uniprot ID P48764) and potentially, these modifications are adding ~10kDa extra to the predicted molecular weight. If you want to confirm it 100%, you could try de-glycosylating your protein and then performing a WB on it. 

Secondary Antibodies

 

Isotype Controls

Additional NHE3/SLC9A3 Products

Research Areas for NHE3/SLC9A3 Antibody (NBP1-46581)

Find related products by research area.

Blogs on NHE3/SLC9A3

There are no specific blogs for NHE3/SLC9A3, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NHE3/SLC9A3 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SLC9A3
Entrez
Uniprot