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NFAT5 Antibody

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Western Blot: NFAT5 Antibody [NBP1-80257] - Host: Rabbit. Target Name: Nfat5. Sample Tissue: Mouse Skeletal Muscle. Antibody Dilution: 3ug/ml
Western Blot: NFAT5 Antibody [NBP1-80257] - SP2/0 cell lysate, concentration 0.2-1 ug/ml.
Western Blot: NFAT5 Antibody [NBP1-80257] - Lanes: Lane 1 : 20 ug HT 29 lysate blocked with 5% FBS Lane 2: 20 ug HT 29 lysate blocked with no FBS Lane 3: 20 ug HT-29 lyaste blocked with no PBS + 100 mM NaCl Primary, ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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NFAT5 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptide directed towards the middle region of mouse NFAT5. Peptide sequence TIQTQDISQPGTFPAVSAASQLPSSDALLQQATQFQTREAQSRDTIQSDT. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFAT5
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
Theoretical MW
166 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for NFAT5 Antibody

  • EC 3.6.1
  • EC 3.6.3.14
  • KIAA0827glutamine rich protein H65
  • NF-AT5NFAT-like protein 1
  • NFATL1
  • NFATZ
  • nuclear factor of activated T-cells 5, tonicity-responsive
  • OREBP
  • osmotic response element-binding protein
  • T-cell transcription factor NFAT5
  • TonE-binding protein
  • TonEBP
  • TONEBPnuclear factor of activated T-cells 5
  • Tonicity-responsive enhancer-binding protein

Background

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NFAT5
Uniprot