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NF-M Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Analysis in human cerebral cortex and endometrium tissues using NBP1-87753 antibody. Corresponding NEFM RNA-seq data are ...read more
Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cell.
Independent Antibodies: Western Blot: NF-M Antibody [NBP1-87753] - Analysis NBP1-87753 (A) shows similar pattern to independent antibody NBP1-87754 (B).
Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NBP1-87753] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human endometrium shows no positivity in glandular cells as expected.
Western Blot: NF-M Antibody [NBP1-87753] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue
Orthogonal Strategies: Western Blot: NF-M Antibody [NBP1-87753] - Analysis in human cell line HEK 293 and human cell line A-431.
Immunocytochemistry/ Immunofluorescence: NF-M Antibody [NBP1-87753] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human cerebral cortex shows strong positivity in neuronal processes.
Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human prostate shows strong positivity in peripheral nerve.
Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87753] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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NF-M Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEEASLVKVELDKKVQSLQDEVAFLRSNH
Marker
Neuronal Marker
Predicted Species
Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NEFM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NF-M Protein (NBP1-87753PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NF-M Antibody

  • NEF3
  • NEF3neurofilament medium polypeptide
  • NEFM
  • Neurofilament 3
  • neurofilament, medium polypeptide 150kDa
  • neurofilament, medium polypeptide
  • neurofilament-3 (150 kD medium)
  • NFM
  • NF-M
  • NF-M160 kDa neurofilament protein
  • NFMNeurofilament triplet M protein

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NEFM