![Western Blot: NEIL2 Antibody (1B7) [H00252969-M01] - Analysis of NEIL2 expression in transfected 293T cell line by NEIL2 monoclonal antibody (M01), clone 1B7.Lane 1: NEIL2 transfected lysate(36.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NEIL2-Antibody-1B7-Western-Blot-H00252969-M01-img0006.jpg "Western Blot: NEIL2 Antibody (1B7) [H00252969-M01] - Analysis of NEIL2 expression in transfected 293T cell line by NEIL2 monoclonal antibody (M01), clone 1B7.Lane 1: NEIL2 transfected lysate(36.8 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, KD |
| Clone | 1B7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | NEIL2 (AAH13964, 1 a.a. ~ 332 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPEGPLVRKFHHLVSPFVGQQVVKTGGSSKKLQPASLQSLWLQDTQVHGKKLFLRFDLDEEMGPPGSSPTPEPPQKEVQKEGAADPKQVGEPSGQKTLDGSSRSAELVPQGEDDSEYLERDAPAGDAGRWLRVSFGLFGSVWVNDFSRAKKANKRGDWRDPSPRLVLHFGGGGFLAFYNCQLSWSSSPVVTPTCDILSEKFHRGQALEALGQAQPVCYTLLDQRYFSGLGNIIKNEALYRAGIHPLSLGSVLSASRREVLVDHVVEFSTAWLQGKFQGRPQHTQVYQKEQCPAGHQVMKEAFGPEDGLQRLTWWCPQCQPQLSEEPEQCQFS |
| Specificity | NEIL2 - nei like 2 (E. coli) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NEIL2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: NEIL2 Antibody (1B7) [H00252969-M01] - Analysis of NEIL2 over-expressed 293 cell line, cotransfected with NEIL2 Validated Chimera RNAi ( Cat # H00252969-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NEIL2 monoclonal antibody (M01), clone 1B7 (Cat # H00252969-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/NEIL2-Antibody-1B7-Western-Blot-H00252969-M01-img0004.jpg "Western Blot: NEIL2 Antibody (1B7) [H00252969-M01] - Analysis of NEIL2 over-expressed 293 cell line, cotransfected with NEIL2 Validated Chimera RNAi ( Cat # H00252969-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NEIL2 monoclonal antibody (M01), clone 1B7 (Cat # H00252969-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![ELISA: NEIL2 Antibody (1B7) [H00252969-M01] - Detection limit for recombinant GST tagged NEIL2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEIL2-Antibody-1B7-ELISA-H00252969-M01-img0007.jpg "ELISA: NEIL2 Antibody (1B7) [H00252969-M01] - Detection limit for recombinant GST tagged NEIL2 is approximately 1ng/ml as a capture antibody.")
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