Name: Nanog Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF2729
Price: 479 USD

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	MuSpecies Glossary
Applications	WB, CyTOF-ready, Flow, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Alexa Fluor 488 IC2729G

Allophycocyanin IC2729A

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Catalog# & Formulation	Size	Price
Carrier Free IC2729A

Carrier Free IC2729G

Carrier Free AF2729-SP

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	IC2729G
Allophycocyanin	IC2729A
Biotin	BAF2729

Nanog Antibody [Unconjugated]

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Nanog Antibody [Unconjugated] Summary

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Publications for Nanog Antibody (AF2729)(35)

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Bioinformatics

Immunogen	E. coli-derived recombinant mouse Nanog Trp154-Leu262 Accession # Q80Z64
Specificity	Detects recombinant mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	NANOG
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Publications using AF2729	Applications	Species
J Sun, S Osenberg, A Irwin, LH Ma, N Lee, Y Xiang, F Li, YW Wan, IH Park, M Maletic-Sa, N Ballas Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation Cell Reports, 2023-01-05;42(1):111942. 2023-01-05 [PMID: 36640327] (IHC, Mouse)	IHC	Mouse
O'Shea TM, Ao Y, Wang S et al. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice Nature Communications 2022-09-28 [PMID: 36171203]
O'Shea TM, Ao Y, Wang S et al. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice Nature Communications 9/28/2022 [PMID: 36171203]
A Fütterer, A Talavera-G, T Pons, J de Celis, J Gutiérrez, V Domínguez, C Martínez-A Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels Cell Death & Disease, 2021-06-21;12(7):637. 2021-06-21 [PMID: 34155199] (IHC, Mouse)	IHC	Mouse
LTP Khoa, YC Tsan, F Mao, DM Kremer, P Sajjakulnu, L Zhang, B Zhou, X Tong, NV Bhanu, C Choudhary, BA Garcia, L Yin, GD Smith, TL Saunders, SL Bielas, CA Lyssiotis, Y Dou Histone Acetyltransferase MOF Blocks Acquisition of Quiescence in Ground-State ESCs through Activating Fatty Acid Oxidation Cell Stem Cell, 2020-06-30;0(0):. 6/30/2020 [PMID: 32610040]
Santosha A. Vardhana, Paige K. Arnold, Bess P. Rosen, Yanyang Chen, Bryce W. Carey, Danwei Huangfu et al. Glutamine independence is a selectable feature of pluripotent stem cells Nature Metabolism 2019-09-13 [PMID: 31511848]
S He, F Wang, Y Zhang, J Chen, L Liang, Y Li, M Zhang, X Yang, H Pang, Y Li, X Huang, D Qin, D Pei, H Sun, H Zheng Hemi-methylated CpG sites connect Dnmt1-knockdown-induced and Tet1-induced DNA demethylation during somatic cell reprogramming Cell Discov, 2019-02-12;5(0):11. 2019-02-12 [PMID: 30774978] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Johanna Kaindl, Ina Meiser, Julia Majer, Annika Sommer, Florian Krach, Alisa Katsen-Globa et al. Zooming in on Cryopreservation of hiPSCs and Neural Derivatives: A Dual-Center Study Using Adherent Vitrification Stem Cells Translational Medicine 2019-03-01 [PMID: 30456912]
NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation Cell Stem Cell, 2018-04-05;22(4):543-558.e12. 2018-04-05 [PMID: 29625069] (ICC, Mouse)	ICC	Mouse
YL Miao, A Gambini, Y Zhang, E Padilla-Ba, WN Jefferson, ML Bernhardt, W Huang, L Li, CJ Williams Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse Biol. Reprod., 2018-04-01;0(0):. 2018-04-01 [PMID: 29325037] (IHC, Mouse)	IHC	Mouse
Xi Chen, Ruizhe Wang, Xu Liu, Yongming Wu, Tao Zhou, Yujia Yang et al. A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3 alpha and GSK3 beta in Regulating Embryonic Stem Cell Fate Developmental Cell 2017-12-04 [PMID: 29207259]
P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Oncotarget, 2017-04-25;8(17):28074-28092. 2017-04-25 [PMID: 28427209] (ICC, Western Blot, Human)	ICC, Western Blot	Human
ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation Stem Cell Res, 2016-08-04;17(2):222-227. 2016-08-04 [PMID: 27591478] (IHC, Human)	IHC	Human
Lysine-specific histone demethylase 1 inhibition promotes reprogramming by facilitating the expression of exogenous transcriptional factors and metabolic switch Sci Rep, 2016-08-02;6(0):30903. 2016-08-02 [PMID: 27481483] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Zhong Liu, Maria Skamagki, Kitai Kim, Rui Zhao Canonical MicroRNA Activity Facilitates but May Be Dispensable for Transcription Factor-Mediated Reprogramming Stem Cell Reports 2015-12-08 [PMID: 26651605]
Friend K, Brooks H, Propson N, Thomson J, Kimble J Embryonic Stem Cell Growth Factors Regulate eIF2alpha Phosphorylation. PLoS ONE, 2015-09-25;10(9):e0139076. 2015-09-25 [PMID: 26406898] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
Hiroyuki Hirai, Meri Firpo, Nobuaki Kikyo Derivation of LIF-independent mouse iPS cells with modified Oct4 Stem Cell Research 2015-09-01 [PMID: 26318720]
Ouyang J, Yu W, Liu J, Zhang N, Florens L, Chen J, Liu H, Washburn M, Pei D, Xie T Cyclin-dependent kinase-mediated Sox2 phosphorylation enhances the ability of Sox2 to establish the pluripotent state. J Biol Chem, 2015-07-02;290(37):22782-94. 2015-07-02 [PMID: 26139602] (ICC, Mouse)	ICC	Mouse
Boulard M, Edwards J, Bestor T FBXL10 protects Polycomb-bound genes from hypermethylation. Nat Genet, 2015-04-06;47(5):479-85. 2015-04-06 [PMID: 25848754] (IHC, Mouse)	IHC	Mouse
Kranc K, Oliveira D, Armesilla-Diaz A, Pacheco-Leyva I, Catarina Matias A, Luisa Escapa A, Subramani C, Wheadon H, Trindade M, Nichols J, Kaji K, Enver T, Braganca J Acute loss of Cited2 impairs Nanog expression and decreases self-renewal of mouse embryonic stem cells. Stem Cells, 2015-03-01;33(3):699-712. 2015-03-01 [PMID: 25377420] (ICC, Western Blot, Mouse)	ICC, Western Blot	Mouse
Alberton P, Dex S, Popov C, Shukunami C, Schieker M, Docheva D Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells. Stem Cells Dev, 2014-12-10;24(5):597-609. 2014-12-10 [PMID: 25351164] (IHC, Mouse)	IHC	Mouse
Zhonghan Li, Jason Dang, Kung-Yen Chang, Tariq M. Rana MicroRNA-mediated regulation of extracellular matrix formation modulates somatic cell reprogramming RNA 2014-12-01 [PMID: 25336587]
Yang , Wei, Lee , Yun-Hwa, Jones , Amanda E, Woolnough , Jessica, Zhou , Dewang, Dai , Qian, Wu , Qiang, Giles , Keith E, Townes , Tim M, Wang , Hengbin The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitment. Nat Commun, 2014-05-02;5(0):3818. 2014-05-02 [PMID: 24784029] (Western Blot, Mouse)	Western Blot	Mouse
Varga E, Nemes C, Davis R, Ujhelly O, Klincumhom N, Polgar Z, Muenthaisong S, Pirity M, Dinnyes A Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system. Exp Cell Res, 2014-02-18;322(2):335-44. 2014-02-18 [PMID: 24560743] (IHC-P, Mouse)	IHC-P	Mouse
Huang G, Yan H, Ye S, Tong C, Ying Q STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates. Stem Cells, 2014-05-01;32(5):1149-60. 2014-05-01 [PMID: 24302476] (Western Blot, Mouse)	Western Blot	Mouse
Tai , Chih-I, Ying , Qi-Long Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state. J Cell Sci, 2013-01-23;126(0):1093-8. 2013-01-23 [PMID: 23345404] (ICC, Mouse)	ICC	Mouse
Zhonghan Li, Tariq M. Rana A kinase inhibitor screen identifies small-molecule enhancers of reprogramming and iPS cell generation Nature Communications 2012-09-25 [PMID: 23011139]
Shai Melcer, Hadas Hezroni, Eyal Rand, Malka Nissim-Rafinia, Arthur Skoultchi, Colin L. Stewart et al. Histone modifications and lamin A regulate chromatin protein dynamics in early embryonic stem cell differentiation Nature Communications 2012-06-19 [PMID: 22713752]
Jesse L. Cox, Sunil K. Mallanna, Briana D. Ormsbee, Michelle Desler, Matthew S. Wiebe, Angie Rizzino Banf1 is required to maintain the self-renewal of both mouse and human embryonic stem cells Journal of Cell Science 2011-08-01 [PMID: 21750191]
Yanzhen Li, Tamaki Yokohama-Tamaki, Tetsuya S. Tanaka Short-Term Serum-Free Culture Reveals that Inhibition of Gsk3 beta Induces the Tumor-Like Growth of Mouse Embryonic Stem Cells PLoS ONE 2011-06-23 [PMID: 21731714]
Li Z, Yang CS, Nakashima K, Rana TM Small RNA-mediated regulation of iPS cell generation. EMBO J., 2011-02-01;30(5):823-34. 2011-02-01 [PMID: 21285944] (ICC, Mouse)	ICC	Mouse
Griffiths DS, Li J, Dawson MA, Trotter MW, Cheng YH, Smith AM, Mansfield W, Liu P, Kouzarides T, Nichols J, Bannister AJ, Green AR, Gottgens B LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell disease. Nat. Cell Biol., 2010-12-12;13(1):13-21. 2010-12-12 [PMID: 21151131] (ICC, Human)	ICC	Human
Guangyu An, Cathrine A. Miner, Jamee C. Nixon, Paul W. Kincade, James Bryant, Philip W. Tucker et al. Loss of Bright/ARID3a Function Promotes Developmental Plasticity Stem Cells 2010-10-26 [PMID: 20680960]
Julia C. Young, Vinali L. Dias, Kate L. Loveland Defining the Window of Germline Genesis In Vitro from Murine Embryonic Stem Cells1 Biology of Reproduction 2010-02-01 [PMID: 19846600]
Parisi S, Passaro F, Aloia L, Manabe I, Nagai R, Pastore L, Russo T Klf5 is involved in self-renewal of mouse embryonic stem cells. J. Cell. Sci., 2008-07-24;121(0):2629-34. 2008-07-24 [PMID: 18653541] (ChIP, ICC, IHC, Mouse)	ChIP, ICC, IHC	Mouse
Show All 35 Publications. Collapse Publications.

Gene Symbol	NANOG
Uniprot	Q80Z64()