![Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Western blot analysis of NALP12 in human brain tissue lysate with NALP12 antibody at 1 u/mL.](http://images.novusbio.com/fullsize/nbp1-76293_rabbit-polyclonal-nalp12-antibody-2711202420414566.gif "Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Western blot analysis of NALP12 in human brain tissue lysate with NALP12 antibody at 1 u/mL.")
| Reactivity | Hu, Mu, Rt, V-ViSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NALP12. The immunogen is located within amino acids 110 - 160 of NALP12. Amino Acid Squence: TPRKDPQETYRDYVRRKF |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NLRP12 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for NALP12 Antibody (NBP1-76293)Find related products by research area.
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Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T... Read full blog post. |
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![Immunohistochemistry-Paraffin: NALP12 Antibody [NBP1-76293] - Human brain tissue. Dilution: 5 ug/mL](http://images.novusbio.com/fullsize/NALP12-Antibody-Immunohistochemistry-Paraffin-NBP1-76293-img0002.jpg "Immunohistochemistry-Paraffin: NALP12 Antibody [NBP1-76293] - Human brain tissue. Dilution: 5 ug/mL")
![Immunocytochemistry/ Immunofluorescence: NALP12 Antibody - BSA Free [NBP1-76293] - Immunofluorescence of NALP12 in human brain tissue with NALP12 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76293_rabbit-polyclonal-nalp12-antibody-2711202420115531.jpg "Immunocytochemistry/ Immunofluorescence: NALP12 Antibody - BSA Free [NBP1-76293] - Immunofluorescence of NALP12 in human brain tissue with NALP12 antibody at 20 u/mL.")
![Western Blot: NALP12 Antibody [NBP1-76293] - B6 mice (n = 6) were infected ocularly with 2.5 x 105 pfu/eye of HSV-1 laboratory strains McKrae, 17, F, RE or KOS or with clinical isolates KOS79 and KOS63 (mock-infected mice (n = 6) used as controls). 48 hrs later, animals were euthanized & corneas were excised & homogenized in lysis buffer, and WB was performed for NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 (beta-actin used as a control). Three WB performed using different corneal lysates harvested from three sets of corneas from 2 mice (4 corneas/lysate). Graphs (mean plus SD) show fold change in expression of NLRP3, NLRP6, NLRP12, IFI16/p204, and AIM2 at 48 hrs post-infection compared to controls, and normalized to beta-actin. Representative densitometric analysis is shown compared to control. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/articles/10.3389/fimmu.2019.01631/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NALP12-Antibody-Western-Blot-NBP1-76293-img0006.jpg "Western Blot: NALP12 Antibody [NBP1-76293] - B6 mice (n = 6) were infected ocularly with 2.5 x 105 pfu/eye of HSV-1 laboratory strains McKrae, 17, F, RE or KOS or with clinical isolates KOS79 and KOS63 (mock-infected mice (n = 6) used as controls). 48 hrs later, animals were euthanized & corneas were excised & homogenized in lysis buffer, and WB was performed for NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 (beta-actin used as a control). Three WB performed using different corneal lysates harvested from three sets of corneas from 2 mice (4 corneas/lysate). Graphs (mean plus SD) show fold change in expression of NLRP3, NLRP6, NLRP12, IFI16/p204, and AIM2 at 48 hrs post-infection compared to controls, and normalized to beta-actin. Representative densitometric analysis is shown compared to control. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/articles/10.3389/fimmu.2019.01631/full), licensed under a CC-BY license.")
![Western Blot: NALP12 Antibody [NBP1-76293] - Expression level of NLRP3, NLRP6, NLRP12, IFI16 and AIM2 inflammasomes in human primary corneal epithelial cells infected with virulent and less-virulent strains of HSV-1. Human primary corneal epithelial cells (hTCEpi cell line) were infected in vitro with clinical isolates KOS79 and KOS63 at MOI of 1. Two, 8 and 24 h post-infection, hTCEpi cells were harvested and immunoblots of whole cell lysates were performed for expression of NLRP3, NLRP6, NLRP12, IFI16, and AIM2 inflammasomes. Corresponding b-actin was used as a control. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2019.01631/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NALP12-Antibody-Western-Blot-NBP1-76293-img0005.jpg "Western Blot: NALP12 Antibody [NBP1-76293] - Expression level of NLRP3, NLRP6, NLRP12, IFI16 and AIM2 inflammasomes in human primary corneal epithelial cells infected with virulent and less-virulent strains of HSV-1. Human primary corneal epithelial cells (hTCEpi cell line) were infected in vitro with clinical isolates KOS79 and KOS63 at MOI of 1. Two, 8 and 24 h post-infection, hTCEpi cells were harvested and immunoblots of whole cell lysates were performed for expression of NLRP3, NLRP6, NLRP12, IFI16, and AIM2 inflammasomes. Corresponding b-actin was used as a control. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2019.01631/full), licensed under a CC-BY license.")
![Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Expression level of NLRP3, NLRP6, NLRP12, IFI16 & AIM2 inflammasomes in human primary corneal epithelial cells infected with virulent & less-virulent strains of HSV-1. Human primary corneal epithelial cells (hTCEpi cell line) were infected in vitro with HSV-1 laboratory strains McKrae, 17, F, RE or KOS (A,B) or with clinical isolates KOS79 & KOS63 (C,D) at MOI of 1. Two, 8 & 24 h post-infection, hTCEpi cells were harvested & immunoblots of whole cell lysates were performed for expression of NLRP3, NLRP6, NLRP12, IFI16, & AIM2 inflammasomes. Corresponding beta -actin was used as a control. (B,D) Graphs show the kinetics of fold changes in NLRP3, NLRP6, NLRP12, IFI16, & AIM2 inflammasomes expression at 2, 8, & 24 h post-infection with virulent/less-virulent strains of HSV-1 compared to the control & normalized to beta -actin. Results are representative of three experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31367214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76293_rabbit-polyclonal-nalp12-antibody-310202416173536.jpg "Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Expression level of NLRP3, NLRP6, NLRP12, IFI16 & AIM2 inflammasomes in human primary corneal epithelial cells infected with virulent & less-virulent strains of HSV-1. Human primary corneal epithelial cells (hTCEpi cell line) were infected in vitro with HSV-1 laboratory strains McKrae, 17, F, RE or KOS (A,B) or with clinical isolates KOS79 & KOS63 (C,D) at MOI of 1. Two, 8 & 24 h post-infection, hTCEpi cells were harvested & immunoblots of whole cell lysates were performed for expression of NLRP3, NLRP6, NLRP12, IFI16, & AIM2 inflammasomes. Corresponding beta -actin was used as a control. (B,D) Graphs show the kinetics of fold changes in NLRP3, NLRP6, NLRP12, IFI16, & AIM2 inflammasomes expression at 2, 8, & 24 h post-infection with virulent/less-virulent strains of HSV-1 compared to the control & normalized to beta -actin. Results are representative of three experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31367214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Expression levels of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 inflammasomes in mouse corneas following infection with virulent & less-virulent strains of HSV-1. B6 mice (n = 6) were infected ocularly with 2.5 × 105 pfu/eye of HSV-1 laboratory strains McKrae, 17, F, RE or KOS or with clinical isolates KOS79 & KOS63. Mock-infected mice (n = 6) were used as controls. Forty-eight hours post-infection animals were euthanized the corneas were excised & homogenized in lysis buffer, & western blot was performed for expression of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2. Corresponding beta -actin was used as a control. Three western blot were performed using three different corneal lysates harvested from three sets of corneas pooled from 2 infected mice (i.e., 4 corneas per lysate). Results are representative of those three independent experiments. Graphs (mean plus SD) show fold change in expression of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 at 48 h post-infection with virulent/less-virulent strains of HSV-1 compared to the control & normalized to beta -actin. Representative densitometric analysis is shown compared to control. *P < 0.05; **P < 0.01; ***P < 0.001 using Student's t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31367214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76293_rabbit-polyclonal-nalp12-antibody-310202416154635.jpg "Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Expression levels of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 inflammasomes in mouse corneas following infection with virulent & less-virulent strains of HSV-1. B6 mice (n = 6) were infected ocularly with 2.5 × 105 pfu/eye of HSV-1 laboratory strains McKrae, 17, F, RE or KOS or with clinical isolates KOS79 & KOS63. Mock-infected mice (n = 6) were used as controls. Forty-eight hours post-infection animals were euthanized the corneas were excised & homogenized in lysis buffer, & western blot was performed for expression of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2. Corresponding beta -actin was used as a control. Three western blot were performed using three different corneal lysates harvested from three sets of corneas pooled from 2 infected mice (i.e., 4 corneas per lysate). Results are representative of those three independent experiments. Graphs (mean plus SD) show fold change in expression of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 at 48 h post-infection with virulent/less-virulent strains of HSV-1 compared to the control & normalized to beta -actin. Representative densitometric analysis is shown compared to control. *P < 0.05; **P < 0.01; ***P < 0.001 using Student's t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31367214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")