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n-Myc Antibody

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Western Blot: n-Myc Antibody [NBP3-13281] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with n-Myc antibody (NBP3-13281) diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more
Western Blot: n-Myc Antibody [NBP3-13281] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with n-Myc antibody (NBP3-13281) diluted at 1:1000. The HRP-conjugated ...read more
Western Blot: n-Myc Antibody [NBP3-13281] - IMR32 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with n-Myc antibody (NBP3-13281) diluted at 1:2500. The ...read more
Western Blot: n-Myc Antibody [NBP3-13281] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with n-Myc antibody (NBP3-13281) diluted at 1:1000. The HRP-conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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n-Myc Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human n-Myc. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYCN
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for n-Myc Antibody

  • BHLHE37
  • bHLHe37N-myc proto-oncogene protein
  • Class E basic helix-loop-helix protein 37
  • MODED
  • neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene
  • N-myc
  • NMYCneuroblastoma MYC oncogene
  • ODED
  • oncogene NMYC
  • pp65/67
  • v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
  • v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)

Background

n-Myc is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB

Publications for n-Myc Antibody (NBP3-13281) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for n-Myc Antibody (NBP3-13281) (0)

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Secondary Antibodies

 

Isotype Controls

Additional n-Myc Products

Research Areas for n-Myc Antibody (NBP3-13281)

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Blogs on n-Myc.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

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Bioinformatics

Gene Symbol MYCN