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MIXL1 Antibody (868132) [Unconjugated]

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MIXL1 was detected in immersion fixed D3 mouse embryonic stem cells differentiated by LIF withdrawal for 3 days using Rat Anti-Mouse MIXL1 Monoclonal Antibody (Catalog # MAB8099) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei in a subset of differentiating cells. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
MIXL1 was detected in immersion fixed D3 mouse embryonic stem cells differentiated by LIF withdrawal for 3 days using Rat Anti-Mouse MIXL1 Monoclonal Antibody (Catalog # MAB8099) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ICC/IF
Clone
868132
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB8099-SP
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MIXL1 Antibody (868132) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse MIXL1
Lys147-Phe231
Accession # Q9WVI0
Specificity
Detects mouse MIXL1 in ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
MIXL1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MIXL1 Antibody (868132) [Unconjugated]

  • hMix
  • homeobox protein MIXL1
  • Homeodomain protein MIX
  • MGC138179
  • MILD1
  • Mix.1 homeobox-like protein
  • Mix1 homeobox (Xenopus laevis)-like 1
  • Mix1 homeobox-like 1 (Xenopus laevis)
  • MIX1 homeobox-like protein 1
  • MIXL1
  • Mix-like homeobox protein 1
  • MIXLMIX

Background

MIXL1 (Mix-like 1) is a 36 kDa member of the paired-like homeodomain protein family. It is expressed in the primitive streak of the gastrulating embryo, and marks cells destined to form mesoderm and endoderm. It is also expressed in differentiating embryonic stem cells, immature B and T lymphocytes during normal hematopoiesis, and several B, T or myeloid lineage acute leukemias. The 231 amino acid (aa) human MIXL1 contains a homeobox domain at aa 86-145.  The mouse MIXL1 immunogen (aa 147-231) shares 72% and 91% aa sequence identity with human and rat MIXL1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MIXL1 Antibody
(868132) [Unconjugate
MAB8099
MIXL1 Antibody (868132) [Unconjugated]
Species: Mu
Applications: ICC/IF

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Bioinformatics

Gene Symbol MIXL1
Uniprot