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Goosecoid Antibody [Unconjugated]

 (8 Publications)
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Goosecoid was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Goosecoid Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4086) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Goosecoid was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Goosecoid Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4086) at 10 µg/mL for 3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF4086
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4086-SP
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Goosecoid Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Goosecoid
Gly48-Arg159
Accession # P56915
Specificity
Detects human Goosecoid in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GSC
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF4086 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goosecoid Antibody [Unconjugated]

  • goosecoid homeobox
  • Goosecoid
  • GSC
  • homeobox protein goosecoid

Background

Goosecoid (GSC) is a 28 kDa member of the paired homeobox family. Human Goosecoid is 257 amino acids (aa) in length, and contains one DNA-binding homeodomain (aa 160‑219). Goosecoid appearance correlates with the start of gastrulation and the development of both head/neck and upper body limb mesenchyme. It also appears abnormally in some tumors where it is associated with an epithelial-to-mesenchymal transition. Human Goosecoid shares 97% aa sequence identity with mouse Goosecoid.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Goosecoid Antibody
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Goosecoid Antibody [Unconjugated]
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Publications for Goosecoid Antibody (AF4086)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, Immunocytochemistry, Western Blot.


Filter By Application
ICC
(3)
Immunocytochemistry
(1)
Western Blot
(2)
All Applications
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Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF4086 Applications Species
Bhattacharya, R;Ward, T;Kalejaiye, TD;Mishra, A;Leeman, S;Arzaghi, H;Seidman, JG;Seidman, CE;Musah, S; Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations bioRxiv : the preprint server for biology 2024-08-06 [PMID: 39211233] (Western Blot, Immunocytochemistry, Human) Western Blot, Immunocytochemistry Human
Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al. Induced hepatic stem cells are suitable for human hepatocyte production iScience 10/21/2022 [PMID: 36147945]
XY Shang, Y Shi, DD He, L Wang, Q Luo, CH Deng, YL Qu, N Wang, ZG Han ARID1A deficiency weakens BRG1-RAD21 interaction that jeopardizes chromatin compactness and drives liver cancer cell metastasis Cell Death & Disease, 2021-10-23;12(11):990. 2021-10-23 [PMID: 34689165] (Western Blot, Mouse) Western Blot Mouse
Jaroslav Fabik, Katarina Kovacova, Zbynek Kozmik, Ondrej Machon Neural crest cells require Meis2 for patterning the mandibular arch via the Sonic hedgehog pathway Biology Open 7/4/2020 [PMID: 32616504]
A Yoney, F Etoc, A Ruzo, T Carroll, JJ Metzger, I Martyn, S Li, C Kirst, ED Siggia, AH Brivanlou WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids Elife, 2018-10-12;7(0):. 2018-10-12 [PMID: 30311909] (ICC, Human) ICC Human
S Musah, N Dimitrakak, DM Camacho, GM Church, DE Ingber Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip Nat Protoc, 2018-07-01;13(7):1662-1685. 2018-07-01 [PMID: 29995874] (ICC, Human) ICC Human
Musah S, Mammoto A, Ferrante TC et al. Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip Nat Biomed Eng. 5/10/2017 [PMID: 29038743]
K Low, LY Wong, M Maldonado, C Manjunath, CB Horner, M Perez, NV Myung, J Nam Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System Stem Cell Reports, 2017-04-27;0(0):. 2017-04-27 [PMID: 28457888] (ICC, Human) ICC Human

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Bioinformatics

Gene Symbol GSC
Uniprot